This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1u8z
From Proteopedia
| Line 4: | Line 4: | ||
|PDB= 1u8z |SIZE=350|CAPTION= <scene name='initialview01'>1u8z</scene>, resolution 1.50Å | |PDB= 1u8z |SIZE=350|CAPTION= <scene name='initialview01'>1u8z</scene>, resolution 1.50Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=GDP:GUANOSINE-5'-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= Rala, Ral-a, Ral ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9490 Saguinus oedipus]) | |GENE= Rala, Ral-a, Ral ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9490 Saguinus oedipus]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1u8y|1U8Y]], [[1u90|1U90]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1u8z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u8z OCA], [http://www.ebi.ac.uk/pdbsum/1u8z PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1u8z RCSB]</span> | ||
}} | }} | ||
| Line 26: | Line 29: | ||
[[Category: Nicely, N I.]] | [[Category: Nicely, N I.]] | ||
[[Category: Serrano, V de.]] | [[Category: Serrano, V de.]] | ||
| - | [[Category: GDP]] | ||
| - | [[Category: MG]] | ||
[[Category: gdp]] | [[Category: gdp]] | ||
[[Category: gmppnp]] | [[Category: gmppnp]] | ||
| Line 37: | Line 38: | ||
[[Category: ra]] | [[Category: ra]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:07:00 2008'' |
Revision as of 21:07, 30 March 2008
| |||||||
| , resolution 1.50Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||
| Gene: | Rala, Ral-a, Ral (Saguinus oedipus) | ||||||
| Related: | 1U8Y, 1U90
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structures of Ral-GppNHp and Ral-GDP reveal two novel binding sites that are also present in Ras and Rap
Overview
RalA is a GTPase with effectors such as Sec5 and Exo84 in the exocyst complex and RalBP1, a GAP for Rho proteins. We report the crystal structures of Ral-GppNHp and Ral-GDP. Disordered switch I and switch II, located away from crystal contacts, are observed in one of the molecules in the asymmetric unit of the Ral-GppNHp structure. In the other molecule in the asymmetric unit, a second Mg(2+) ion is bound to the GppNHp gamma-phosphate in an environment in which switch I is pulled away from the nucleotide and switch II is found in a tight beta turn. Clustering of conserved residues on the surface of Ral-GppNHp identifies two putative sites for protein-protein interaction. One site is adjacent to switch I. The other is modulated by switch II and is obstructed in Ral-GDP. The Ral structures are discussed in the context of the published structures of the Ral/Sec5 complex, Ras, and Rap.
About this Structure
1U8Z is a Single protein structure of sequence from Saguinus oedipus. Full crystallographic information is available from OCA.
Reference
Crystal structures of Ral-GppNHp and Ral-GDP reveal two binding sites that are also present in Ras and Rap., Nicely NI, Kosak J, de Serrano V, Mattos C, Structure. 2004 Nov;12(11):2025-36. PMID:15530367
Page seeded by OCA on Mon Mar 31 00:07:00 2008
Categories: Saguinus oedipus | Single protein | Kosak, J. | Mattos, C. | Nicely, N I. | Serrano, V de. | Gdp | Gmppnp | Gnp | Gppnhp | Gtp | Gtpase | Ral | Ra
