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5uzm
From Proteopedia
(Difference between revisions)
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5uzm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5uzm OCA], [http://pdbe.org/5uzm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5uzm RCSB], [http://www.ebi.ac.uk/pdbsum/5uzm PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5uzm ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5uzm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5uzm OCA], [http://pdbe.org/5uzm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5uzm RCSB], [http://www.ebi.ac.uk/pdbsum/5uzm PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5uzm ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The Drosophila hnRNP F/H homolog, Glorund (Glo), regulates nanos mRNA translation by interacting with a structured UA-rich motif in the nanos 3' untranslated region. Glo regulates additional RNAs, however, and mammalian homologs bind G-tract sequences to regulate alternative splicing, suggesting that Glo also recognizes G-tract RNA. To gain insight into how Glo recognizes both structured UA-rich and G-tract RNAs, we used mutational analysis guided by crystal structures of Glo's RNA-binding domains and identified two discrete RNA-binding surfaces that allow Glo to recognize both RNA motifs. By engineering Glo variants that favor a single RNA-binding mode, we show that a subset of Glo's functions in vivo is mediated solely by the G-tract binding mode, whereas regulation of nanos requires both recognition modes. Our findings suggest a molecular mechanism for the evolution of dual RNA motif recognition in Glo that may be applied to understanding the functional diversity of other RNA-binding proteins. | ||
| + | |||
| + | The Drosophila hnRNP F/H Homolog Glorund Uses Two Distinct RNA-Binding Modes to Diversify Target Recognition.,Tamayo JV, Teramoto T, Chatterjee S, Hall TM, Gavis ER Cell Rep. 2017 Apr 4;19(1):150-161. doi: 10.1016/j.celrep.2017.03.022. PMID:28380354<ref>PMID:28380354</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 5uzm" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Revision as of 06:24, 19 April 2017
Crystal structure of Glorund qRRM2 domain
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