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1uix

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|PDB= 1uix |SIZE=350|CAPTION= <scene name='initialview01'>1uix</scene>, resolution 1.8&Aring;
|PDB= 1uix |SIZE=350|CAPTION= <scene name='initialview01'>1uix</scene>, resolution 1.8&Aring;
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1uix FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uix OCA], [http://www.ebi.ac.uk/pdbsum/1uix PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1uix RCSB]</span>
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[[Category: coiled-coil]]
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Revision as of 21:10, 30 March 2008

Image:1uix.jpg


PDB ID 1uix

Drag the structure with the mouse to rotate
, resolution 1.8Å
Ligands:
Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



Coiled-coil structure of the RhoA-binding domain in Rho-kinase


Overview

Rho-kinase is a serine/threonine protein kinase that regulates cytoskeletal events in cells. The enzyme activity of Rho-kinase is auto-inhibited in the free state but is activated through direct binding to the small GTPase Rho in the GTP-bound form. The crystal structure of the Rho-binding domain (RhoBD) of Rho-kinase has been determined at 1.8-A resolution by the multi-wavelength anomalous dispersion technique. The structure shows that RhoBD dimerizes to form a parallel coiled-coil with long consecutive alpha-helices extended to approximately 97 A and suggests that free Rho-kinase can also form a dimer through parallel self-association. At the middle region of the coiled-coil, the polypeptide chains are flexible and display loose "knobs-into-holes" packing of the side chains from both chains. RhoBD residues that have been shown to be critical for Rho-binding are spread in the positively charged C-terminal region. The parallel coiled-coil structure of our Rho-kinase RhoBD in the free form is different from the anti-parallel coiled-coil structure of RhoBD of protein kinase N when complexed with RhoA. Implications derived from these structural studies in relation to the mechanism of Rho-kinase activation will be addressed with previously reported experimental data.

About this Structure

1UIX is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.

Reference

Parallel coiled-coil association of the RhoA-binding domain in Rho-kinase., Shimizu T, Ihara K, Maesaki R, Amano M, Kaibuchi K, Hakoshima T, J Biol Chem. 2003 Nov 14;278(46):46046-51. Epub 2003 Sep 3. PMID:12954645

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