1umz
From Proteopedia
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|PDB= 1umz |SIZE=350|CAPTION= <scene name='initialview01'>1umz</scene>, resolution 1.80Å | |PDB= 1umz |SIZE=350|CAPTION= <scene name='initialview01'>1umz</scene>, resolution 1.80Å | ||
|SITE= <scene name='pdbsite=AC1:Gal+Binding+Site+For+Chain+B'>AC1</scene> | |SITE= <scene name='pdbsite=AC1:Gal+Binding+Site+For+Chain+B'>AC1</scene> | ||
| - | |LIGAND= | + | |LIGAND= <scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=UNK:UNKNOWN'>UNK</scene>, <scene name='pdbligand=XYS:XYLOPYRANOSE'>XYS</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Xyloglucan:xyloglucosyl_transferase Xyloglucan:xyloglucosyl transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.207 2.4.1.207] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Xyloglucan:xyloglucosyl_transferase Xyloglucan:xyloglucosyl transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.207 2.4.1.207] </span> |
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1umz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1umz OCA], [http://www.ebi.ac.uk/pdbsum/1umz PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1umz RCSB]</span> | ||
}} | }} | ||
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[[Category: xyloglucan]] | [[Category: xyloglucan]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:12:31 2008'' |
Revision as of 21:12, 30 March 2008
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| , resolution 1.80Å | |||||||
|---|---|---|---|---|---|---|---|
| Sites: | |||||||
| Ligands: | , , , , , | ||||||
| Activity: | Xyloglucan:xyloglucosyl transferase, with EC number 2.4.1.207 | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
XYLOGLUCAN ENDOTRANSGLYCOSYLASE IN COMPLEX WITH THE XYLOGLUCAN NONASACCHARIDE XLLG.
Overview
Xyloglucan endotransglycosylases (XETs) cleave and religate xyloglucan polymers in plant cell walls via a transglycosylation mechanism. Thus, XET is a key enzyme in all plant processes that require cell wall remodeling. To provide a basis for detailed structure-function studies, the crystal structure of Populus tremula x tremuloides XET16A (PttXET16A), heterologously expressed in Pichia pastoris, has been determined at 1.8-A resolution. Even though the overall structure of PttXET16A is a curved beta-sandwich similar to other enzymes in the glycoside hydrolase family GH16, parts of its substrate binding cleft are more reminiscent of the distantly related family GH7. In addition, XET has a C-terminal extension that packs against the conserved core, providing an additional beta-strand and a short alpha-helix. The structure of XET in complex with a xyloglucan nonasaccharide, XLLG, reveals a very favorable acceptor binding site, which is a necessary but not sufficient prerequisite for transglycosylation. Biochemical data imply that the enzyme requires sugar residues in both acceptor and donor sites to properly orient the glycosidic bond relative to the catalytic residues.
About this Structure
1UMZ is a Single protein structure of sequence from Populus tremula. Full crystallographic information is available from OCA.
Reference
Crystal structures of a poplar xyloglucan endotransglycosylase reveal details of transglycosylation acceptor binding., Johansson P, Brumer H 3rd, Baumann MJ, Kallas AM, Henriksson H, Denman SE, Teeri TT, Jones TA, Plant Cell. 2004 Apr;16(4):874-86. Epub 2004 Mar 12. PMID:15020748
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