User:Luke Edward Severinac/Sandbox 1

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=='''Phosphorylation'''==
=='''Phosphorylation'''==
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The function of Caspase-6 can be inhibited by phosphorylation of Ser-257. The exact mechanism of this reaction remains unidentified at the time of publication, but proceeds when ARK5 kinase is present. This modification can occur before and after zymogen activation. The phosphoryl group inhibits Caspase-6 through steric interference. When Ser-257 is phosphorylated, the amino acid residue interacts with <scene name='75/752344/Caspase-6_his-208/1'>Pro-201</scene>, causing a shift in the helices of Caspase-6<ref name="ActRegofCas6inND">PMID: 25340928 </ref>. This is shown in the <scene name='75/752344/Caspase-6_s257d_mutant/1'>S257D Caspase-6 mutant</scene>, whose mutation mimics phosphorylation<ref name="Phosregcasp6subsbindgroove">PMID: 22483120 </ref>. The shift misaligns and disrupts residues found in the active site. This conformational difference prevents the intersubunit linker from entering during zymogen activation and the self-cleaved active dimer cannot be formed. Additionally, no new substrate is able to enter the active site.
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The function of Caspase-6 can be inhibited by phosphorylation of Ser-257. The exact mechanism of this reaction remains unidentified at the time of publication, but proceeds when ARK5 kinase is present. This modification can occur before and after zymogen activation. The phosphoryl group inhibits Caspase-6 through steric interference. When Ser-257 is phosphorylated, the amino acid residue interacts with <scene name='75/752344/Caspase-6_his-208/1'>Pro-201</scene>, causing a shift in the helices of Caspase-6<ref name="ActRegofCas6inND">PMID: 25340928 </ref>. This is shown in the <scene name='75/752344/Caspase-6_s257d_mutantboi/1'>S257D Caspase-6 mutant</scene>, whose mutation mimics phosphorylation<ref name="Phosregcasp6subsbindgroove">PMID: 22483120 </ref>. The shift misaligns and disrupts residues found in the active site. This conformational difference prevents the intersubunit linker from entering during zymogen activation and the self-cleaved active dimer cannot be formed. Additionally, no new substrate is able to enter the active site.
=='''Medical Relevance'''==
=='''Medical Relevance'''==

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Caspase-6 in Homo sapiens

Caspase-6

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