5x7g

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'''Unreleased structure'''
 
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The entry 5x7g is ON HOLD until Paper Publication
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==Crystal Structure of Paenibacillus sp. 598K cycloisomaltooligosaccharide glucanotransferase==
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<StructureSection load='5x7g' size='340' side='right' caption='[[5x7g]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[5x7g]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5X7G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5X7G FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MLI:MALONATE+ION'>MLI</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5x7h|5x7h]], [[5x7o|5x7o]], [[5x7p|5x7p]], [[5x7q|5x7q]], [[5x7r|5x7r]], [[5x7s|5x7s]]</td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cycloisomaltooligosaccharide_glucanotransferase Cycloisomaltooligosaccharide glucanotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.248 2.4.1.248] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5x7g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5x7g OCA], [http://pdbe.org/5x7g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5x7g RCSB], [http://www.ebi.ac.uk/pdbsum/5x7g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5x7g ProSAT]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Paenibacillus sp. 598K cycloisomaltooligosaccharide glucanotransferase (PsCITase), a member of glycoside hydrolase family 66, catalyzes the intramolecular transglucosylation of dextran to produce cycloisomaltooligosaccharides (CIs) with seven or more degrees of polymerization. To clarify the cyclization reaction and product specificity of the enzyme, we determined the crystal structure of PsCITase. The core structure of PsCITase consists of four structural domains: a catalytic (beta/alpha)8-domain and three beta-domains. A family 35 carbohydrate-binding module (PsCBM35-1) is inserted into and protrudes from the catalytic domain. The ligand complex structure of PsCITase prepared by soaking the crystal with cycloisomaltoheptaose yielded bound sugars at three sites: in the catalytic cleft; at the joint of the PsCBM35-1 domain; and at the loop region of PsCBM35-1. In the catalytic site, soaked cycloisomaltoheptaose was observed as a linear isomaltoheptaose, presumably a hydrolyzed product from cycloisomaltoheptaose by the enzyme, and occupied subsites -7 to -1. Beyond subsite -7, three glucose moieties of another isomaltooiligosaccharide were observed, and these positions are considered to be distal subsites -11 to -13. The third binding site is the canonical sugar-binding site at the loop region of PsCBM35-1, where the soaked cycloisomaltoheptaose was bound. The structure indicated that the concave surface between the catalytic domain and PsCBM35-1 plays a guiding route for the long-chained substrate at the cyclization reaction.
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Authors: Fujimoto, Z., Kishine, N., Suzuki, N., Suzuki, R., Momma, M., Funane, K.
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Isomaltooligosaccharide-binding structure of Paenibacillus sp. 598K cycloisomaltooligosaccharide glucanotransferase.,Fujimoto Z, Kishine N, Suzuki N, Suzuki R, Mizushima D, Momma M, Kimura K, Funane K Biosci Rep. 2017 Apr 6. pii: BSR20170253. doi: 10.1042/BSR20170253. PMID:28385816<ref>PMID:28385816</ref>
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Description: Crystal Structure of Paenibacillus sp. 598K cycloisomaltooligosaccharide glucanotransferase
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Unreleased Structures]]
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</div>
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<div class="pdbe-citations 5x7g" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Cycloisomaltooligosaccharide glucanotransferase]]
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[[Category: Fujimoto, Z]]
[[Category: Funane, K]]
[[Category: Funane, K]]
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[[Category: Suzuki, R]]
 
[[Category: Kishine, N]]
[[Category: Kishine, N]]
[[Category: Momma, M]]
[[Category: Momma, M]]
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[[Category: Fujimoto, Z]]
 
[[Category: Suzuki, N]]
[[Category: Suzuki, N]]
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[[Category: Suzuki, R]]
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[[Category: Carbohydrate-binding module family 35]]
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[[Category: Glydoside hydrolase family 66]]
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[[Category: Transferase]]

Revision as of 13:51, 27 April 2017

Crystal Structure of Paenibacillus sp. 598K cycloisomaltooligosaccharide glucanotransferase

5x7g, resolution 2.20Å

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