1uwv
From Proteopedia
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|PDB= 1uwv |SIZE=350|CAPTION= <scene name='initialview01'>1uwv</scene>, resolution 1.95Å | |PDB= 1uwv |SIZE=350|CAPTION= <scene name='initialview01'>1uwv</scene>, resolution 1.95Å | ||
|SITE= <scene name='pdbsite=CAT:Po4+Binding+Site+For+Chain+A'>CAT</scene> | |SITE= <scene name='pdbsite=CAT:Po4+Binding+Site+For+Chain+A'>CAT</scene> | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1uwv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uwv OCA], [http://www.ebi.ac.uk/pdbsum/1uwv PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1uwv RCSB]</span> | ||
}} | }} | ||
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[[Category: Lee, T T.]] | [[Category: Lee, T T.]] | ||
[[Category: Stroud, R M.]] | [[Category: Stroud, R M.]] | ||
| - | [[Category: CL]] | ||
| - | [[Category: NI]] | ||
| - | [[Category: PO4]] | ||
| - | [[Category: SF4]] | ||
[[Category: iron-sulfur cluster]] | [[Category: iron-sulfur cluster]] | ||
[[Category: methyltransferase]] | [[Category: methyltransferase]] | ||
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[[Category: transferase]] | [[Category: transferase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:16:25 2008'' |
Revision as of 21:16, 30 March 2008
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| , resolution 1.95Å | |||||||
|---|---|---|---|---|---|---|---|
| Sites: | |||||||
| Ligands: | , , , , | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE OF RUMA, THE IRON-SULFUR CLUSTER CONTAINING E. COLI 23S RIBOSOMAL RNA 5-METHYLURIDINE METHYLTRANSFERASE
Overview
RumA catalyzes transfer of a methyl group from S-adenosylmethionine (SAM) specifically to uridine 1939 of 23S ribosomal RNA in Escherichia coli to yield 5-methyluridine. We determined the crystal structure of RumA at 1.95 A resolution. The protein is organized into three structural domains: The N-terminal domain contains sequence homology to the conserved TRAM motif and displays a five-stranded beta barrel architecture characteristic of an oligosaccharide/oligonucleotide binding fold. The central domain contains a [Fe(4)S(4)] cluster coordinated by four conserved cysteine residues. The C-terminal domain displays the typical SAM-dependent methyltransferase fold. The catalytic nucleophile Cys389 lies in a motif different from that in DNA 5-methylcytosine methyltransferases. The electrostatic potential surface reveals a predominately positively charged area that covers the concave surface of the first two domains and suggests an RNA binding mode. The iron-sulfur cluster may be involved in the correct folding of the protein or may have a role in RNA binding.
About this Structure
1UWV is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Crystal structure of RumA, an iron-sulfur cluster containing E. coli ribosomal RNA 5-methyluridine methyltransferase., Lee TT, Agarwalla S, Stroud RM, Structure. 2004 Mar;12(3):397-407. PMID:15016356
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