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4xhr

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Revision as of 13:20, 10 May 2017

Structure of a phospholipid trafficking complex, native

Structural highlights

4xhr is a 4 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	4xiz
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[UPS1_YEAST] Required for maintenance of normal mitochondrial morphology as well as PCP1-dependent processing of MGM1. With UPS2, controls the level of cardiolipin in mitochondria. Cardiolipin is a unique phospholipid with four fatty acid chains and is present mainly in the mitochondrial inner membrane where it stabilizes the electron transport chain supercomplex between complexes III and IV through direct interaction of their subunits.^[1] ^[2] ^[3] ^[4] [MDM35_YEAST] Involved in mitochondrial distribution and morphology. Mediates the import of UPS1, UPS2 and UPS3, 3 atypical mitochondrial intermembrane space (IMS) proteins lacking the two major IMS-targeting signals, into the intermembrane space.^[5] ^[6] ^[7]

Publication Abstract from PubMed

Ups1 forms a complex with Mdm35 and is critical for the transport of phosphatidic acid (PA) from the mitochondrial outer membrane to the inner membrane. We report the crystal structure of the Ups1-Mdm35-PA complex and the functional characterization of Ups1-Mdm35 in PA binding and transfer. Ups1 features a barrel-like structure consisting of an antiparallel beta-sheet and three alpha-helices. Mdm35 adopts a three-helical clamp-like structure to wrap around Ups1 to form a stable complex. The beta-sheet and alpha-helices of Ups1 form a long tunnel-like pocket to accommodate the substrate PA, and a short helix alpha2 acts as a lid to cover the pocket. The hydrophobic residues lining the pocket and helix alpha2 are critical for PA binding and transfer. In addition, a hydrophilic patch on the surface of Ups1 near the PA phosphate-binding site also plays an important role in the function of Ups1-Mdm35. Our study reveals the molecular basis of the function of Ups1-Mdm35 and sheds new light on the mechanism of intramitochondrial phospholipid transport by the MSF1/PRELI family proteins.

Structural basis of intramitochondrial phosphatidic acid transport mediated by Ups1-Mdm35 complex.,Yu F, He F, Yao H, Wang C, Wang J, Li J, Qi X, Xue H, Ding J, Zhang P EMBO Rep. 2015 Jun 12. pii: e201540137. PMID:26071601^[8]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Sesaki H, Dunn CD, Iijima M, Shepard KA, Yaffe MP, Machamer CE, Jensen RE. Ups1p, a conserved intermembrane space protein, regulates mitochondrial shape and alternative topogenesis of Mgm1p. J Cell Biol. 2006 Jun 5;173(5):651-8. PMID:16754953 doi:http://dx.doi.org/10.1083/jcb.200603092
↑ Osman C, Haag M, Potting C, Rodenfels J, Dip PV, Wieland FT, Brugger B, Westermann B, Langer T. The genetic interactome of prohibitins: coordinated control of cardiolipin and phosphatidylethanolamine by conserved regulators in mitochondria. J Cell Biol. 2009 Feb 23;184(4):583-96. doi: 10.1083/jcb.200810189. Epub 2009 Feb, 16. PMID:19221197 doi:http://dx.doi.org/10.1083/jcb.200810189
↑ Tamura Y, Endo T, Iijima M, Sesaki H. Ups1p and Ups2p antagonistically regulate cardiolipin metabolism in mitochondria. J Cell Biol. 2009 Jun 15;185(6):1029-45. Epub 2009 Jun 8. PMID:19506038 doi:http://dx.doi.org/jcb.200812018
↑ Tamura Y, Iijima M, Sesaki H. Mdm35p imports Ups proteins into the mitochondrial intermembrane space by functional complex formation. EMBO J. 2010 Sep 1;29(17):2875-87. doi: 10.1038/emboj.2010.149. Epub 2010 Jul 9. PMID:20622808 doi:http://dx.doi.org/10.1038/emboj.2010.149
↑ Dimmer KS, Fritz S, Fuchs F, Messerschmitt M, Weinbach N, Neupert W, Westermann B. Genetic basis of mitochondrial function and morphology in Saccharomyces cerevisiae. Mol Biol Cell. 2002 Mar;13(3):847-53. PMID:11907266 doi:10.1091/mbc.01-12-0588
↑ Tamura Y, Iijima M, Sesaki H. Mdm35p imports Ups proteins into the mitochondrial intermembrane space by functional complex formation. EMBO J. 2010 Sep 1;29(17):2875-87. doi: 10.1038/emboj.2010.149. Epub 2010 Jul 9. PMID:20622808 doi:http://dx.doi.org/10.1038/emboj.2010.149
↑ Potting C, Wilmes C, Engmann T, Osman C, Langer T. Regulation of mitochondrial phospholipids by Ups1/PRELI-like proteins depends on proteolysis and Mdm35. EMBO J. 2010 Sep 1;29(17):2888-98. doi: 10.1038/emboj.2010.169. Epub 2010 Jul 23. PMID:20657548 doi:http://dx.doi.org/10.1038/emboj.2010.169
↑ Yu F, He F, Yao H, Wang C, Wang J, Li J, Qi X, Xue H, Ding J, Zhang P. Structural basis of intramitochondrial phosphatidic acid transport mediated by Ups1-Mdm35 complex. EMBO Rep. 2015 Jun 12. pii: e201540137. PMID:26071601 doi:http://dx.doi.org/10.15252/embr.201540137

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4xhr"

@@ Line 1: / Line 1: @@
 ==Structure of a phospholipid trafficking complex, native==
 <StructureSection load='4xhr' size='340' side='right' caption='[[4xhr]], [[Resolution|resolution]] 2.55&Aring;' scene=''>
@@ Line 4: / Line 5: @@
 <table><tr><td colspan='2'>[[4xhr]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XHR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4XHR FirstGlance]. <br>
 </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4xiz|4xiz]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4xhr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xhr OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4xhr RCSB], [http://www.ebi.ac.uk/pdbsum/4xhr PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4xhr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xhr OCA], [http://pdbe.org/4xhr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4xhr RCSB], [http://www.ebi.ac.uk/pdbsum/4xhr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4xhr ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 16: / Line 17: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 4xhr" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>

4xhr

From Proteopedia

Revision as of 13:20, 10 May 2017

Structure of a phospholipid trafficking complex, native

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

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