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4z8k

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Revision as of 16:05, 17 May 2017

Phycocyanin structure from T. elongatus at 2.5-A from XFEL using a viscous delivery medium for serial femtosecond crystallography

Structural highlights

4z8k is a 2 chain structure with sequence from Thermosynechococcus elongatus bp-1. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
NonStd Res:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[PHCA_THEEB] Light-harvesting photosynthetic bile pigment-protein from the phycobiliprotein complex. [PHCB_THEEB] Light-harvesting photosynthetic bile pigment-protein from the phycobiliprotein complex.

Publication Abstract from PubMed

Serial femtosecond crystallography (SFX) has opened a new era in crystallo-graphy by permitting nearly damage-free, room-temperature structure determination of challenging proteins such as membrane proteins. In SFX, femtosecond X-ray free-electron laser pulses produce diffraction snapshots from nanocrystals and microcrystals delivered in a liquid jet, which leads to high protein consumption. A slow-moving stream of agarose has been developed as a new crystal delivery medium for SFX. It has low background scattering, is compatible with both soluble and membrane proteins, and can deliver the protein crystals at a wide range of temperatures down to 4 degrees C. Using this crystal-laden agarose stream, the structure of a multi-subunit complex, phycocyanin, was solved to 2.5 A resolution using 300 microg of microcrystals embedded into the agarose medium post-crystallization. The agarose delivery method reduces protein consumption by at least 100-fold and has the potential to be used for a diverse population of proteins, including membrane protein complexes.

A novel inert crystal delivery medium for serial femtosecond crystallography.,Conrad CE, Basu S, James D, Wang D, Schaffer A, Roy-Chowdhury S, Zatsepin NA, Aquila A, Coe J, Gati C, Hunter MS, Koglin JE, Kupitz C, Nelson G, Subramanian G, White TA, Zhao Y, Zook J, Boutet S, Cherezov V, Spence JC, Fromme R, Weierstall U, Fromme P IUCrJ. 2015 Jun 30;2(Pt 4):421-30. doi: 10.1107/S2052252515009811. eCollection, 2015 Jul 1. PMID:26177184^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Conrad CE, Basu S, James D, Wang D, Schaffer A, Roy-Chowdhury S, Zatsepin NA, Aquila A, Coe J, Gati C, Hunter MS, Koglin JE, Kupitz C, Nelson G, Subramanian G, White TA, Zhao Y, Zook J, Boutet S, Cherezov V, Spence JC, Fromme R, Weierstall U, Fromme P. A novel inert crystal delivery medium for serial femtosecond crystallography. IUCrJ. 2015 Jun 30;2(Pt 4):421-30. doi: 10.1107/S2052252515009811. eCollection, 2015 Jul 1. PMID:26177184 doi:http://dx.doi.org/10.1107/S2052252515009811

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4z8k"

@@ Line 1: / Line 1: @@
 ==Phycocyanin structure from T. elongatus at 2.5-A from XFEL using a viscous delivery medium for serial femtosecond crystallography==
 <StructureSection load='4z8k' size='340' side='right' caption='[[4z8k]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
@@ Line 5: / Line 6: @@
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CYC:PHYCOCYANOBILIN'>CYC</scene></td></tr>
 <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MEN:N-METHYL+ASPARAGINE'>MEN</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4z8k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4z8k OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4z8k RCSB], [http://www.ebi.ac.uk/pdbsum/4z8k PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4z8k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4z8k OCA], [http://pdbe.org/4z8k PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4z8k RCSB], [http://www.ebi.ac.uk/pdbsum/4z8k PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4z8k ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 17: / Line 18: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 4z8k" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>

4z8k

From Proteopedia

Revision as of 16:05, 17 May 2017

Phycocyanin structure from T. elongatus at 2.5-A from XFEL using a viscous delivery medium for serial femtosecond crystallography

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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