This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
5u66
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
| - | '''Unreleased structure''' | ||
| - | + | ==Modified single helix from the B-domain of protein A bound to IgG1 Fc== | |
| + | <StructureSection load='5u66' size='340' side='right' caption='[[5u66]], [[Resolution|resolution]] 1.70Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5u66]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5U66 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5U66 FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | ||
| + | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=85G:N-ETHYL-L-GLUTAMINE'>85G</scene>, <scene name='pdbligand=85J:N-PROPYL-L-GLUTAMINE'>85J</scene>, <scene name='pdbligand=SIN:SUCCINIC+ACID'>SIN</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5u66 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5u66 OCA], [http://pdbe.org/5u66 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5u66 RCSB], [http://www.ebi.ac.uk/pdbsum/5u66 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5u66 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The well-studied B-domain from Staphylococcal protein A is a 59 amino acid three-helix bundle that binds the Fc portion of IgG with a dissociation constant of ~35 nM. The B-domain variant bearing a Gly to Ala mutation (=Z-domain) has been the subject of efforts to minimize a domain's size while retaining its function. We report X-ray crystallographic characterization of three steps in such a process using complexes with Fc: the full three-helix Z-domain, a 34 amino acid two-helix version called Z34C and a 13 amino acid single helix stabilized with an exo-helix tether, called LH1. | ||
| - | + | 3-2-1: Structural insights from stepwise shrinkage of a three-helix Fc-binding domain to a single helix.,Ultsch M, Braisted A, Maun HR, Eigenbrot C Protein Eng Des Sel. 2017 May 5:1-7. doi: 10.1093/protein/gzx029. PMID:28475752<ref>PMID:28475752</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 5u66" style="background-color:#fffaf0;"></div> | |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Eigenbrot, C]] | [[Category: Eigenbrot, C]] | ||
| + | [[Category: Ultsch, M H]] | ||
| + | [[Category: B-domain]] | ||
| + | [[Category: Igg1 fc]] | ||
| + | [[Category: Immune system-inhibitor complex]] | ||
| + | [[Category: Protein some]] | ||
| + | [[Category: Staple peptide]] | ||
Revision as of 13:40, 24 May 2017
Modified single helix from the B-domain of protein A bound to IgG1 Fc
| |||||||||||
