4wih

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Revision as of 14:14, 24 May 2017

Crystal structure of cAMP-dependent Protein Kinase A from Cricetulus griseus

Structural highlights

4wih is a 2 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
NonStd Res:	,
Activity:	cAMP-dependent protein kinase, with EC number 2.7.11.11
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[KAPCA_CRIGR] Phosphorylates a large number of substrates in the cytoplasm and the nucleus. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis. Phosphorylates CDC25B, ABL1, NFKB1, CLDN3, PSMC5/RPT6, PJA2, RYR2, RORA and VASP. RORA is activated by phosphorylation. Required for glucose-mediated adipogenic differentiation increase and osteogenic differentiation inhibition from osteoblasts. Involved in the regulation of platelets in response to thrombin and collagen; maintains circulating platelets in a resting state by phosphorylating proteins in numerous platelet inhibitory pathways when in complex with NF-kappa-B (NFKB1 and NFKB2) and I-kappa-B-alpha (NFKBIA), but thrombin and collagen disrupt these complexes and free active PRKACA stimulates platelets and leads to platelet aggregation by phosphorylating VASP. Prevents the antiproliferative and anti-invasive effects of alpha-difluoromethylornithine in breast cancer cells when activated. RYR2 channel activity is potentiated by phosphorylation in presence of luminal Ca(2+), leading to reduced amplitude and increased frequency of store overload-induced Ca(2+) release (SOICR) characterized by an increased rate of Ca(2+) release and propagation velocity of spontaneous Ca(2+) waves, despite reduced wave amplitude and resting cytosolic Ca(2+). PSMC5/RPT6 activation by phosphorylation stimulates proteasome. Negatively regulates tight junctions (TJs) in ovarian cancer cells via CLDN3 phosphorylation. NFKB1 phosphorylation promotes NF-kappa-B p50-p50 DNA binding. Involved in embryonic development by down-regulating the Hedgehog (Hh) signaling pathway that determines embryo pattern formation and morphogenesis. Prevents meiosis resumption in prophase-arrested oocytes via CDC25B inactivation by phosphorylation. May also regulate rapid eye movement (REM) sleep in the pedunculopontine tegmental (PPT) (By similarity). Phosphorylates APOBEC3G and AICDA (By similarity).

Publication Abstract from PubMed

Protein kinases (PKs) are dynamic regulators of numerous cellular processes. Their phosphorylation activity is determined by the conserved kinase core structure, which is maintained by the interaction and dynamics with associated domains or interacting proteins. The prototype enzyme for investigations to understand the activity and regulation of PKs is the catalytic subunit of cAMP-dependent protein kinase (PKAc). Major effects of functional regulation and ligand binding are driven by only minor structural modulations in protein-protein interactions. In order to resolve such minor structural differences, very high resolution structures are required. Here, the high-resolution X-ray structure of PKAc from Cricetulus griseus is reported.

High-resolution crystal structure of cAMP-dependent protein kinase from Cricetulus griseus.,Kudlinzki D, Linhard VL, Saxena K, Sreeramulu S, Gande S, Schieborr U, Dreyer M, Schwalbe H Acta Crystallogr F Struct Biol Commun. 2015 Aug 1;71(Pt 8):1088-93. doi:, 10.1107/S2053230X1501242X. Epub 2015 Jul 29. PMID:26249705^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Kudlinzki D, Linhard VL, Saxena K, Sreeramulu S, Gande S, Schieborr U, Dreyer M, Schwalbe H. High-resolution crystal structure of cAMP-dependent protein kinase from Cricetulus griseus. Acta Crystallogr F Struct Biol Commun. 2015 Aug 1;71(Pt 8):1088-93. doi:, 10.1107/S2053230X1501242X. Epub 2015 Jul 29. PMID:26249705 doi:http://dx.doi.org/10.1107/S2053230X1501242X

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4wih"

@@ Line 1: / Line 1: @@
 ==Crystal structure of cAMP-dependent Protein Kinase A from Cricetulus griseus==
 <StructureSection load='4wih' size='340' side='right' caption='[[4wih]], [[Resolution|resolution]] 1.14&Aring;' scene=''>
@@ Line 5: / Line 6: @@
 </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene>, <scene name='pdbligand=TPO:PHOSPHOTHREONINE'>TPO</scene></td></tr>
 <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/cAMP-dependent_protein_kinase cAMP-dependent protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.11 2.7.11.11] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wih FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wih OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4wih RCSB], [http://www.ebi.ac.uk/pdbsum/4wih PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wih FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wih OCA], [http://pdbe.org/4wih PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4wih RCSB], [http://www.ebi.ac.uk/pdbsum/4wih PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4wih ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 17: / Line 18: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 4wih" style="background-color:#fffaf0;"></div>
 ==See Also==

4wih

From Proteopedia

Revision as of 14:14, 24 May 2017

Crystal structure of cAMP-dependent Protein Kinase A from Cricetulus griseus

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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