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GP1 of Lassa Virus

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[https://en.wikipedia.org/wiki/X-ray_crystallography X-ray diffraction]
[https://en.wikipedia.org/wiki/X-ray_crystallography X-ray diffraction]
=== Histidine Triad===
=== Histidine Triad===
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Attached to this structure is a <scene name='76/761695/Histriad/5'>unique triad of histidines</scene> that is highly conserved among Old World arenaviruses. Located on the β-sheet face of GP1, the histidine triad is a structural element that directly interacts with [[LAMP1]] and helps stabilize a LAMP1-"compatible" conformation by providing a molecular mechanism for the pH-dependent receptor switching<ref name="MolMechforLAMP1" />. The binding of LAMP1 triggers the glycoprotein spike complex of LASV to catalyze [https://en.wikipedia.org/wiki/Lipid_bilayer_fusion membrane fusion] by potentiating its response to pH<ref name="RoleofLAMP1BindingandpHsensing">DOI 10.1128/JVI.01624-16</ref>.
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Attached to this structure is a <scene name='76/761695/Histriad/5'>unique triad of histidines</scene> that is highly conserved among Old World arenaviruses. Located on the β-sheet face of GP1, the histidine triad is a structural element that directly interacts with [[LAMP1]] (Lysosome-associated membrane glycoprotein) and helps stabilize a LAMP1-"compatible" conformation by providing a molecular mechanism for the pH-dependent receptor switching<ref name="MolMechforLAMP1" />.
===LAMP1 Binding Site===
===LAMP1 Binding Site===
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Recent studies show that receptor switching is critical during cell entry of LASV. Receptor switching to LAMP1 is critical for triggering membrane fusion by LASV.
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To effectively infect cells, LASV needs to switch in an endosomal compartment from its primary receptor, [[α-dystroglycan]] (α-DG), to LAMP1<ref name="RoleofLAMP1BindingandpHsensing">DOI 10.1128/JVI.01624-16</ref>. Recent studies show that receptor switching is critical during cell entry of LASV.
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The binding of LAMP1 triggers the glycoprotein spike complex of LASV to catalyze [https://en.wikipedia.org/wiki/Lipid_bilayer_fusion membrane fusion] by potentiating its response to pH.
Protein structure accession number: '''4ZJF'''
Protein structure accession number: '''4ZJF'''

Revision as of 07:57, 27 June 2017

4ZJF structure

Drag the structure with the mouse to rotate

References

  1. ↑ 1.0 1.1 Cohen-Dvashi H, Cohen N, Israeli H, Diskin R. Molecular mechanism for LAMP1 recognition by Lassa Virus. J Virol. 2015 May 13. pii: JVI.00651-15. PMID:25972533 doi:http://dx.doi.org/10.1128/JVI.00651-15
  2. ↑ Cohen-Dvashi H, Israeli H, Shani O, Katz A, Diskin R. Role of LAMP1 Binding and pH Sensing by the Spike Complex of Lassa Virus. J Virol. 2016 Oct 28;90(22):10329-10338. Print 2016 Nov 15. PMID:27605678 doi:http://dx.doi.org/10.1128/JVI.01624-16

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