User:Wayne Decatur/Sequence analysis tools

From Proteopedia

Revision as of 16:22, 5 July 2017

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• Plasmapper
• Online Schematic plasmid drawing tool
• Online restriction mapper
• NEBcutter
• old web cutter
• Sequence Manipulation Suite: Restriction Map - has at the left side links to other tools they have
• Biotools at UMASS MED (formerly included EMBOSS
• `cons` alignment consensus program and many others at EMBOSS explorer website
• Links to many EMBOSS portals, servers and mirrors under 'Servers'
• MUSCLE: MUltiple Sequence Comparison by Log-Expectation
• Archaeopteryx for the visualization of annotated phylogenetic trees.
• Netprimer
• Genomicus: Genomes in Evolution - "genome browser that enables users to navigate in genomes in several dimensions: linearly along chromosome axes, transversaly across different species, and chronologically along evolutionary time."
• SeqTrace- "is an application for viewing and processing DNA sequencing chromatograms (trace files). SeqTrace makes it easy to quickly generate high-quality finished sequences from a large number of trace files. SeqTrace can automatically identify, align, and compute [contig] consensus sequences from matching forward and reverse traces, filter low-quality base calls, and perform end trimming of finished sequences. The finished DNA sequences can then be exported to common sequence file formats, such as FASTA. " Written in Python.
• CAP3 Sequence Assembly Program - online, webserver for making contigs from DNA sequences. "form allows you to assemble a set of contiguous sequences (contigs) with the CAP3 program.
• Nucleobytes - DNA editor and 4peaks sequence chromatogram viewer along with other mac software
• PaxDb: Protein Abundance Across Organisms
• PrePPI: database of predicted and experimentally determined protein-protein interactions (PPIs) for yeast and human.
• T-profiler - for scoring the activity Of pre-defined groups of yeast genes using gene expression data **As of May 2016 it was not accepting uploads.**
• g:Profiler - for characterizing and manipulating gene lists of high-throughput genomics. Handles yeast and many other organisms.
• ProViz - a web-based visualization tool to investigate the functional and evolutionary features of protein sequences.

Converters

• ALTER (ALignment Transformation EnviRonment) - complex interface but offers lots of options for output. I used it as part of my workflow to get closer to special NEXUS format (or intermediate) for performing maximum likelihood phylogenetic analysis of large sets of sequences.
• Sequence conversion Provided by bugaco.com - a lot of conversion choices with easy interface. When I had interleaved clustal format it converted nicely to a straight fasta listing for the sequence for every organism.
• Reformat utility of Max Planck Institute for Developmental Biology Bioinformatics Toolkit converts sequences or multiple sequence alignments to various forms.
• Three to One converts three letter amino acid sequence translations to single letter translations.
• One to Three converts single letter amino acid sequence translations to three letter translations.
• ConvertSeq folder at github - my own converter scripts.
• g:Convert - Gene ID Converter. Handles yeast and a very large list of other organisms.

Random sequence generators

• http://users-birc.au.dk/biopv/php/fabox/random_sequence_generator.php
• http://www.bioinformatics.org/sms2/random_dna.html
• http://www.faculty.ucr.edu/~mmaduro/random.htm
• http://molbiol.ru/eng/scripts/01_16.html

Sequence shufflers

• http://emboss.sourceforge.net/ - shuffleseq from EMBOSS shuffles a set of sequences maintaining composition.

Orthology

• EggNOG - A database of orthologous groups and functional annotation

Some sequence analysis but mostly OTHER

• BioCyc Database Collection - "BioCyc is a collection of 3530 Pathway/Genome Databases (PGDBs), with tools for understanding their data. Cellular Overview image generated by Pathway Tools. Explore Metabolic Maps for Thousands of Organisms. RouteSearch: Search for Paths through the Metabolic Network. Cross-Organism Search form generated by Pathway Tools. New: Search All of BioCyc for Genes, Proteins, Pathways. Search all of BioCyc or designated taxonomic groups for named genes, proteins, metabolites, pathways. Multiple Sequence Alignment results generated by Pathway Tools using MUSCLE. PatMatch query and results by Pathway Tools. SmartTable display generated by Pathway Tools. Metabolomics Data Analysis. Cellular Overview Omics Viewer image generated by Pathway Tools. Gene Expression Data Analysis. Multi-Genome Browser. Comparative Genome Analysis."

Good E. coli database

• - EcoProDB E. coli protein database (EcoProDB) integrates protein information identified on 2-D gels along with other resources to provide the comparative platform for the expression levels of many heterogeneous proteins under different genetic and environmental conditions using the interactive interface and search mechanism.

NGS

• HOMER - "Software for motif discovery and next-gen sequencing analysis". Nice in that it actually explains some of the details and advantages of the browsers and file types.

Nucleic acid system building

• NUPACK - "NUPACK is a growing software suite for the analysis and design of nucleic acid systems."

Fungal Genome Resources

http://fungalgenomes.org/

http://1000.fungalgenomes.org/home/

http://fungidb.org/fungidb/ (about it –> http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3245123/)

http://genome.jgi.doe.gov/programs/fungi/1000fungalgenomes.jsf <— nice graphic of situation related to 1000 fungal genomes project

http://genome.jgi-psf.org/programs/fungi/index.jsf

http://www.broadinstitute.org/scientific-community/science/projects/fungal-genome-initiative/fungal-genomics

http://fungi.ensembl.org/index.html

http://en.wikipedia.org/wiki/List_of_sequenced_fungi_genomes <– how current is it???

For genomic arrangement (synteny) comparisons/Fungal Genomics Resources

Synteny Viewer listed under every SGD gene on Sequence tab, near bottom of page

http://www.genomicus.biologie.ens.fr/genomicus-fungi-19.01/cgi-bin/search.pl

Yeast Gene Order Browser (YGOB)

RNA Structure Analysis

• Infernal - A downloadable program for equence analysis using profiles of RNA sequence based on Rfam-associated covariance models and secondary structure consensus. The program can generate covariance models from RNA alignments as well. Binaries are avialble for Mac, Windows, and Linux. ( E. P. Nawrocki and S. R. Eddy, Infernal 1.1: 100-fold faster RNA homology searches , Bioinformatics 29:2933-2935 (2013). PMID: 24008419)

Sequence Logo Generation

• WebLogo
• Seq2Logo
• Skylign
• Generating publication-ready sequence logos in R using ggseqlogo

Installable software for fine-tuning sequence alignments

• SEQOTRON - Mac Software for adjusting sequence alignments by hand. Unfortunately it discards the conservation data if it is there in input. Haven't found a way to put it back in the output other than use `cons` alignment consensus program and many others at EMBOSS explorer website

My own sequence work-related code

• Sequence manipulation Python code
• Working with UGENE software analysis software
• Working with Yeastmine