5uk6
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Structure of Anabaena Sensory Rhodopsin Determined by Solid State NMR Spectroscopy and DEER== | |
| + | <StructureSection load='5uk6' size='340' side='right' caption='[[5uk6]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5uk6]] is a 3 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UK6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5UK6 FirstGlance]. <br> | ||
| + | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=LYR:N~6~-[(2Z,4E,6E,8E)-3,7-DIMETHYL-9-(2,6,6-TRIMETHYLCYCLOHEX-1-EN-1-YL)NONA-2,4,6,8-TETRAENYL]LYSINE'>LYR</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5uk6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5uk6 OCA], [http://pdbe.org/5uk6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5uk6 RCSB], [http://www.ebi.ac.uk/pdbsum/5uk6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5uk6 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Oligomerization of membrane proteins is common in nature. Here, we combine spin-labeling double electron-electron resonance (DEER) and solid-state NMR (ssNMR) spectroscopy to refine the structure of an oligomeric integral membrane protein, Anabaena sensory rhodopsin (ASR), reconstituted in a lipid environment. An essential feature of such a combined approach is that it provides structural distance restraints spanning a range of ca 3-60A while using the same sample preparation (i.e., mutations, paramagnetic labeling, and reconstitution in lipid bilayers) for both ssNMR and DEER. Direct modeling of the multispin effects on DEER signal allowed for the determination of the oligomeric order and for obtaining long-range DEER distance restraints between the ASR trimer subunits that were used to refine the ssNMR structure of ASR. The improved structure of the ASR trimer revealed a more compact packing of helices and side chains at the intermonomer interface, compared to the structure determined using the ssNMR data alone. The extent of the refinement is significant when compared with typical helix movements observed for the active states of homologous proteins. Our combined approach of using complementary DEER and NMR measurements for the determination of oligomeric structures would be widely applicable to membrane proteins where paramagnetic tags can be introduced. Such a method could be used to study the effects of the lipid membrane composition on protein oligomerization and to observe structural changes in protein oligomers upon drug, substrate, and co-factor binding. | ||
| - | + | Oligomeric Structure of Anabaena Sensory Rhodopsin in a Lipid Bilayer Environment by Combining Solid-State NMR and Long-range DEER Constraints.,Milikisiyants S, Wang S, Munro RA, Donohue M, Ward ME, Bolton D, Brown LS, Smirnova TI, Ladizhansky V, Smirnov AI J Mol Biol. 2017 Jun 16;429(12):1903-1920. doi: 10.1016/j.jmb.2017.05.005. Epub, 2017 May 10. PMID:28501588<ref>PMID:28501588</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 5uk6" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Brown, L S]] | ||
| + | [[Category: Donohue, M]] | ||
| + | [[Category: Ladizhansky, V]] | ||
| + | [[Category: Milikisiyants, S]] | ||
| + | [[Category: Munro, R A]] | ||
| + | [[Category: Smirnov, A I]] | ||
| + | [[Category: Smirnova, T I]] | ||
| + | [[Category: Wang, S]] | ||
| + | [[Category: Ward, M E]] | ||
| + | [[Category: Anabeana sensory rhodopsin]] | ||
| + | [[Category: Seven-transmembrane protein]] | ||
| + | [[Category: Signaling protein]] | ||
| + | [[Category: Trimer]] | ||
Revision as of 08:00, 3 August 2017
Structure of Anabaena Sensory Rhodopsin Determined by Solid State NMR Spectroscopy and DEER
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