This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
5ii6
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of the ZP-N1 domain of mouse sperm receptor ZP2 at 0.95 A resolution== | |
| + | <StructureSection load='5ii6' size='340' side='right' caption='[[5ii6]], [[Resolution|resolution]] 0.95Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5ii6]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5II6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5II6 FirstGlance]. <br> | ||
| + | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3d4c|3d4c]], [[3d4g|3d4g]], [[3ef7|3ef7]], [[3nk3|3nk3]], [[3nk4|3nk4]], [[5bup|5bup]]</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ii6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ii6 OCA], [http://pdbe.org/5ii6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ii6 RCSB], [http://www.ebi.ac.uk/pdbsum/5ii6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ii6 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/ZP2_MOUSE ZP2_MOUSE]] The mammalian zona pellucida, which mediates species-specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy, is composed of three to four glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP2 may act as a secondary sperm receptor.<ref>PMID:22472438</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Recognition between sperm and the egg surface marks the beginning of life in all sexually reproducing organisms. This fundamental biological event depends on the species-specific interaction between rapidly evolving counterpart molecules on the gametes. We report biochemical, crystallographic, and mutational studies of domain repeats 1-3 of invertebrate egg coat protein VERL and their interaction with cognate sperm protein lysin. VERL repeats fold like the functionally essential N-terminal repeat of mammalian sperm receptor ZP2, whose structure is also described here. Whereas sequence-divergent repeat 1 does not bind lysin, repeat 3 binds it non-species specifically via a high-affinity, largely hydrophobic interface. Due to its intermediate binding affinity, repeat 2 selectively interacts with lysin from the same species. Exposure of a highly positively charged surface of VERL-bound lysin suggests that complex formation both disrupts the organization of egg coat filaments and triggers their electrostatic repulsion, thereby opening a hole for sperm penetration and fusion. | ||
| - | + | Structural Basis of Egg Coat-Sperm Recognition at Fertilization.,Raj I, Sadat Al Hosseini H, Dioguardi E, Nishimura K, Han L, Villa A, de Sanctis D, Jovine L Cell. 2017 Jun 15;169(7):1315-1326.e17. doi: 10.1016/j.cell.2017.05.033. PMID:28622512<ref>PMID:28622512</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 5ii6" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Dioguardi, E]] | ||
| + | [[Category: Han, L]] | ||
| + | [[Category: Jovine, L]] | ||
| + | [[Category: Nishimura, K]] | ||
| + | [[Category: Sanctis, D De]] | ||
| + | [[Category: Cell adhesion]] | ||
| + | [[Category: Egg coat]] | ||
| + | [[Category: Egg-sperm interaction]] | ||
| + | [[Category: Fertilization]] | ||
| + | [[Category: Gamete recognition]] | ||
| + | [[Category: Sperm receptor]] | ||
| + | [[Category: Zona pellucida]] | ||
| + | [[Category: Zp domain]] | ||
| + | [[Category: Zp-n domain]] | ||
Revision as of 09:00, 3 August 2017
Crystal structure of the ZP-N1 domain of mouse sperm receptor ZP2 at 0.95 A resolution
| |||||||||||
