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2jcd

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Revision as of 09:26, 3 August 2017

Structure of the N-oxygenase AurF from Streptomyces thioluteus

Structural highlights

2jcd is a 2 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Nitro groups are found in a number of bioactive compounds. Most of them arise by a stepwise mono-oxygenation of amino groups. One of the involved enzymes is AurF participating in the biosynthesis of aureothin. Its structure was established at 2.1 A resolution showing a homodimer with a binuclear manganese cluster. The enzyme preparation, which yielded the analyzed crystals, showed activity using in vitro and in vivo assays. Chain fold and cluster are homologous with ribonucleotide reductase subunit R2 and related enzymes. The two manganese ions and an iron content of about 15% were established by anomalous X-ray diffraction. A comparison of the cluster with more common di-iron clusters suggested an additional histidine in the coordination sphere to cause the preference for manganese over iron. There is no oxo-bridge. The substrate p-amino-benzoate was modeled into the active center. The model is supported by mutant activity measurements. It shows the geometry of the reaction and explains the established substrate spectrum.

Structure and action of the N-oxygenase AurF from Streptomyces thioluteus.,Zocher G, Winkler R, Hertweck C, Schulz GE J Mol Biol. 2007 Oct 12;373(1):65-74. Epub 2007 Jun 9. PMID:17765264^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Zocher G, Winkler R, Hertweck C, Schulz GE. Structure and action of the N-oxygenase AurF from Streptomyces thioluteus. J Mol Biol. 2007 Oct 12;373(1):65-74. Epub 2007 Jun 9. PMID:17765264 doi:10.1016/j.jmb.2007.06.014

1 Structural highlights
2 Evolutionary Conservation
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2jcd"

@@ Line 1: / Line 1: @@
-==STRUCTURE OF THE N-OXYGENASE AURF FROM STREPTOMYCES THIOLUTEUS==
+==Structure of the N-oxygenase AurF from Streptomyces thioluteus==
 <StructureSection load='2jcd' size='340' side='right' caption='[[2jcd]], [[Resolution|resolution]] 2.11&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2jcd]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"streptomyces_thioluteus"_okami_1952 "streptomyces thioluteus" okami 1952]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JCD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2JCD FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2jcd]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JCD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2JCD FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jcd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jcd OCA], [http://pdbe.org/2jcd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2jcd RCSB], [http://www.ebi.ac.uk/pdbsum/2jcd PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jcd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jcd OCA], [http://pdbe.org/2jcd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2jcd RCSB], [http://www.ebi.ac.uk/pdbsum/2jcd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2jcd ProSAT]</span></td></tr>
 </table>
 == Evolutionary Conservation ==
@@ Line 29: / Line 30: @@
 __TOC__
 </StructureSection>
-[[Category: Streptomyces thioluteus okami 1952]]
 [[Category: Hertweck, C]]
 [[Category: Schulz, G E]]

2jcd

From Proteopedia

Revision as of 09:26, 3 August 2017

Structure of the N-oxygenase AurF from Streptomyces thioluteus

Structural highlights

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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