5ii7

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'''Unreleased structure'''
 
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The entry 5ii7 is ON HOLD until Paper Publication
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==In-house sulfur-SAD structure of orthorhombic red abalone lysin at 1.66 A resolution==
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<StructureSection load='5ii7' size='340' side='right' caption='[[5ii7]], [[Resolution|resolution]] 1.66&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[5ii7]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5II7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5II7 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1lis|1lis]], [[2lis|2lis]], [[1lyn|1lyn]], [[2lyn|2lyn]], [[5ii8|5ii8]], [[5ii9|5ii9]], [[5iia|5iia]]</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ii7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ii7 OCA], [http://pdbe.org/5ii7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ii7 RCSB], [http://www.ebi.ac.uk/pdbsum/5ii7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ii7 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[[http://www.uniprot.org/uniprot/ELYS_HALRU ELYS_HALRU]] Dissolves the egg vitelline layer nonenzymatically during fertilization. It creates a hole of about 3 mu-m in diameter through which the sperm pass.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Recognition between sperm and the egg surface marks the beginning of life in all sexually reproducing organisms. This fundamental biological event depends on the species-specific interaction between rapidly evolving counterpart molecules on the gametes. We report biochemical, crystallographic, and mutational studies of domain repeats 1-3 of invertebrate egg coat protein VERL and their interaction with cognate sperm protein lysin. VERL repeats fold like the functionally essential N-terminal repeat of mammalian sperm receptor ZP2, whose structure is also described here. Whereas sequence-divergent repeat 1 does not bind lysin, repeat 3 binds it non-species specifically via a high-affinity, largely hydrophobic interface. Due to its intermediate binding affinity, repeat 2 selectively interacts with lysin from the same species. Exposure of a highly positively charged surface of VERL-bound lysin suggests that complex formation both disrupts the organization of egg coat filaments and triggers their electrostatic repulsion, thereby opening a hole for sperm penetration and fusion.
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Authors:
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Structural Basis of Egg Coat-Sperm Recognition at Fertilization.,Raj I, Sadat Al Hosseini H, Dioguardi E, Nishimura K, Han L, Villa A, de Sanctis D, Jovine L Cell. 2017 Jun 15;169(7):1315-1326.e17. doi: 10.1016/j.cell.2017.05.033. PMID:28622512<ref>PMID:28622512</ref>
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Description:
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Unreleased Structures]]
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</div>
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<div class="pdbe-citations 5ii7" style="background-color:#fffaf0;"></div>
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==See Also==
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*[[Lysin|Lysin]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Al-Hosseini, H Sadat]]
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[[Category: Jovine, L]]
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[[Category: Nishimura, K]]
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[[Category: Raj, I]]
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[[Category: Acrosomal protein]]
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[[Category: Cell adhesion]]
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[[Category: Crystal dehydration]]
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[[Category: Egg coat penetration]]
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[[Category: Egg-binding protein]]
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[[Category: Egg-sperm interaction]]
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[[Category: Fertilization]]
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[[Category: Gamete recognition]]
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[[Category: S-sad]]
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[[Category: Sulfur-sad]]

Revision as of 10:15, 3 August 2017

In-house sulfur-SAD structure of orthorhombic red abalone lysin at 1.66 A resolution

5ii7, resolution 1.66Å

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