2bxx

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(Difference between revisions)

Revision as of 10:22, 3 August 2017

Crystal structure of the N-terminal domain of IBV coronavirus nucleocapsid. Native crystal form

Structural highlights

2bxx is a 2 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	2btl
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The coronavirus nucleocapsid (N) protein packages viral genomic RNA into a ribonucleoprotein complex. Interactions between N proteins and RNA are thus crucial for the assembly of infectious virus particles. The 45 kDa recombinant nucleocapsid N protein of coronavirus infectious bronchitis virus (IBV) is highly sensitive to proteolysis. We obtained a stable fragment of 14.7 kDa spanning its N-terminal residues 29-160 (IBV-N29-160). Like the N-terminal RNA binding domain (SARS-N45-181) of the severe acute respiratory syndrome virus (SARS-CoV) N protein, the crystal structure of the IBV-N29-160 fragment at 1.85 A resolution reveals a protein core composed of a five-stranded antiparallel beta sheet with a positively charged beta hairpin extension and a hydrophobic platform that are probably involved in RNA binding. Crosslinking studies demonstrate the formation of dimers, tetramers, and higher multimers of IBV-N. A model for coronavirus shell formation is proposed in which dimerization of the C-terminal domain of IBV-N leads to oligomerization of the IBV-nucleocapsid protein and viral RNA condensation.

The nucleocapsid protein of coronavirus infectious bronchitis virus: crystal structure of its N-terminal domain and multimerization properties.,Fan H, Ooi A, Tan YW, Wang S, Fang S, Liu DX, Lescar J Structure. 2005 Dec;13(12):1859-68. PMID:16338414^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Fan H, Ooi A, Tan YW, Wang S, Fang S, Liu DX, Lescar J. The nucleocapsid protein of coronavirus infectious bronchitis virus: crystal structure of its N-terminal domain and multimerization properties. Structure. 2005 Dec;13(12):1859-68. PMID:16338414 doi:10.1016/j.str.2005.08.021

1 Structural highlights
2 Evolutionary Conservation
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2bxx"

@@ Line 1: / Line 1: @@
-==CRYSTAL STRUCTURE OF THE N-TERMINAL DOMAIN OF IBV CORONAVIRUS NUCLEOCAPSID. NATIVE CRYSTAL FORM==
+==Crystal structure of the N-terminal domain of IBV coronavirus nucleocapsid. Native crystal form==
 <StructureSection load='2bxx' size='340' side='right' caption='[[2bxx]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2bxx]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Avian_infectious_bronchitis_virus Avian infectious bronchitis virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BXX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BXX FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2bxx]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BXX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BXX FirstGlance]. <br>
 </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2btl|2btl]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bxx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bxx OCA], [http://pdbe.org/2bxx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2bxx RCSB], [http://www.ebi.ac.uk/pdbsum/2bxx PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bxx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bxx OCA], [http://pdbe.org/2bxx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2bxx RCSB], [http://www.ebi.ac.uk/pdbsum/2bxx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2bxx ProSAT]</span></td></tr>
 </table>
 == Evolutionary Conservation ==
@@ Line 14: / Line 15: @@
     <text>to colour the structure by Evolutionary Conservation</text>
   </jmolCheckbox>
-</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2bxx ConSurf].
 <div style="clear:both"></div>
 <div style="background-color:#fffaf0;">
@@ Line 32: / Line 33: @@
 __TOC__
 </StructureSection>
-[[Category: Avian infectious bronchitis virus]]
 [[Category: Fan, H]]
 [[Category: Lescar, J]]

2bxx

From Proteopedia

Revision as of 10:22, 3 August 2017

Crystal structure of the N-terminal domain of IBV coronavirus nucleocapsid. Native crystal form

Structural highlights

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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