5myy
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Hen Egg-White Lysozyme (HEWL) cocrystallized in the presence of Cadmium sulphate== | |
| + | <StructureSection load='5myy' size='340' side='right' caption='[[5myy]], [[Resolution|resolution]] 1.10Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5myy]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MYY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5MYY FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | ||
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5myy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5myy OCA], [http://pdbe.org/5myy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5myy RCSB], [http://www.ebi.ac.uk/pdbsum/5myy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5myy ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK]] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Cadmium ions can be effectively used to promote crystal growth and for experimental phasing. Here, the use of cadmium ions as a suitable anomalous scatterer at the standard wavelength of 1 A is demonstrated. The structures of three different proteins were determined using cadmium single-wavelength anomalous dispersion (SAD) phasing. Owing to the strong anomalous signal, the structure of lysozyme could be automatically phased and built using a very low anomalous multiplicity (1.1) and low-completeness (77%) data set. Additionally, it is shown that cadmium ions can easily substitute divalent ions in ATP-divalent cation complexes. This property could be generally applied for phasing experiments of a wide range of nucleotide-binding proteins. Improvements in crystal growth and quality, good anomalous signal at standard wavelengths (i.e. no need to change photon energy) and rapid phasing and refinement using a single data set are benefits that should allow cadmium ions to be widely used for experimental phasing. | ||
| - | + | Rapid cadmium SAD phasing at the standard wavelength (1 A).,Panneerselvam S, Kumpula EP, Kursula I, Burkhardt A, Meents A Acta Crystallogr D Struct Biol. 2017 Jul 1;73(Pt 7):581-590. doi:, 10.1107/S2059798317006970. Epub 2017 Jun 30. PMID:28695858<ref>PMID:28695858</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 5myy" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Lysozyme]] | ||
| + | [[Category: Burkhardt, A]] | ||
| + | [[Category: Meents, A]] | ||
| + | [[Category: Panneerselvam, S]] | ||
| + | [[Category: Cadmium phasing]] | ||
| + | [[Category: Hydrolase]] | ||
| + | [[Category: Sad phasing]] | ||
Revision as of 04:01, 4 August 2017
Hen Egg-White Lysozyme (HEWL) cocrystallized in the presence of Cadmium sulphate
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