1w0j
From Proteopedia
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|PDB= 1w0j |SIZE=350|CAPTION= <scene name='initialview01'>1w0j</scene>, resolution 2.20Å | |PDB= 1w0j |SIZE=350|CAPTION= <scene name='initialview01'>1w0j</scene>, resolution 2.20Å | ||
|SITE= <scene name='pdbsite=PLP:Bef+Binding+Site+For+Chain+F'>PLP</scene> | |SITE= <scene name='pdbsite=PLP:Bef+Binding+Site+For+Chain+F'>PLP</scene> | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=ADP:ADENOSINE-5'-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=BEF:BERYLLIUM+TRIFLUORIDE+ION'>BEF</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/H(+)-transporting_two-sector_ATPase H(+)-transporting two-sector ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.14 3.6.3.14] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/H(+)-transporting_two-sector_ATPase H(+)-transporting two-sector ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.14 3.6.3.14] </span> |
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1w0j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1w0j OCA], [http://www.ebi.ac.uk/pdbsum/1w0j PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1w0j RCSB]</span> | ||
}} | }} | ||
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[[Category: Montgomery, M G.]] | [[Category: Montgomery, M G.]] | ||
[[Category: Walker, J E.]] | [[Category: Walker, J E.]] | ||
| - | [[Category: ADP]] | ||
| - | [[Category: BEF]] | ||
| - | [[Category: GOL]] | ||
| - | [[Category: MG]] | ||
| - | [[Category: PO4]] | ||
[[Category: atp phosphorylase]] | [[Category: atp phosphorylase]] | ||
[[Category: atp phosphorylase (h+ transporting)]] | [[Category: atp phosphorylase (h+ transporting)]] | ||
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[[Category: hydrolase]] | [[Category: hydrolase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:29:28 2008'' |
Revision as of 21:29, 30 March 2008
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| , resolution 2.20Å | |||||||
|---|---|---|---|---|---|---|---|
| Sites: | |||||||
| Ligands: | , , , , | ||||||
| Activity: | H(+)-transporting two-sector ATPase, with EC number 3.6.3.14 | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
BERYLLIUM FLUORIDE INHIBITED BOVINE F1-ATPASE
Overview
The structure of bovine F1-ATPase inhibited with ADP and beryllium fluoride at 2.0 angstroms resolution contains two ADP.BeF3- complexes mimicking ATP, bound in the catalytic sites of the beta(TP) and beta(DP) subunits. Except for a 1 angstrom shift in the guanidinium of alphaArg373, the conformations of catalytic side chains are very similar in both sites. However, the ordered water molecule that carries out nucleophilic attack on the gamma-phosphate of ATP during hydrolysis is 2.6 angstroms from the beryllium in the beta(DP) subunit and 3.8 angstroms away in the beta(TP) subunit, strongly indicating that the beta(DP) subunit is the catalytically active conformation. In the structure of F1-ATPase with five bound ADP molecules (three in alpha-subunits, one each in the beta(TP) and beta(DP) subunits), which has also been determined, the conformation of alphaArg373 suggests that it senses the presence (or absence) of the gamma-phosphate of ATP. Two catalytic schemes are discussed concerning the various structures of bovine F1-ATPase.
About this Structure
1W0J is a Protein complex structure of sequences from Bos taurus. Full crystallographic information is available from OCA.
Reference
The structure of bovine F1-ATPase inhibited by ADP and beryllium fluoride., Kagawa R, Montgomery MG, Braig K, Leslie AG, Walker JE, EMBO J. 2004 Jul 21;23(14):2734-44. Epub 2004 Jul 1. PMID:15229653
Page seeded by OCA on Mon Mar 31 00:29:28 2008
Categories: Bos taurus | H(+)-transporting two-sector ATPase | Protein complex | Braig, K. | Kagawa, R. | Leslie, A G.W. | Montgomery, M G. | Walker, J E. | Atp phosphorylase | Atp phosphorylase (h+ transporting) | Atp synthase | Atp synthesis | Atp-binding | F1-atpase | F1fo atp synthase | Hydrolase
