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5vmd
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of UBR-box from UBR6 in a domain-swapping conformation== | |
| + | <StructureSection load='5vmd' size='340' side='right' caption='[[5vmd]], [[Resolution|resolution]] 2.20Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5vmd]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VMD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5VMD FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5vmd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vmd OCA], [http://pdbe.org/5vmd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5vmd RCSB], [http://www.ebi.ac.uk/pdbsum/5vmd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5vmd ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Disease == | ||
| + | [[http://www.uniprot.org/uniprot/FBX11_HUMAN FBX11_HUMAN]] Vitiligo. Defects in FBXO11 may be a cause of diffuse large B-cell lymphoma by allowing the accumulation of BCL6, an oncoprotein that has a critical role in lymphomas.<ref>PMID:22113614</ref> | ||
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/FBX11_HUMAN FBX11_HUMAN]] Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins, such as DTL/CDT2, BCL6 and PRDM1/BLIMP1. The SCF(FBXO11) complex mediates ubiquitination and degradation of BCL6, thereby playing a role in the germinal center B-cells terminal differentiation toward memory B-cells and plasma cells. The SCF(FBXO11) complex also mediates ubiquitination and degradation of DTL, an important step for the regulation of TGF-beta signaling, cell migration and the timing of the cell-cycle progression and exit. Binds to and neddylates phosphorylated p53/TP53, inhibiting its transcriptional activity. SCF(FBXO11) does not seem to direct ubiquitination of p53/TP53.<ref>PMID:17098746</ref> <ref>PMID:22113614</ref> <ref>PMID:23478441</ref> <ref>PMID:23478445</ref> <ref>PMID:23892434</ref> <ref>PMID:24613396</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The UBR-box is a 70-residue zinc finger domain present in the UBR family of E3 ubiquitin ligases that directly binds N-terminal degradation signals in substrate proteins. UBR6, also called FBXO11, is an UBR-box containing E3 ubiquitin ligase that does not bind N-terminal signals. Here, we present the crystal structure of the UBR-box domain from human UBR6. The dimeric crystal structure reveals a unique form of domain swapping mediated by zinc coordination, where three independent protein chains come together to regenerate the topology of the monomeric UBR-box fold. Analysis of the structure suggests that the absence of N-terminal residue binding arises from the lack of an amino acid binding pocket. | ||
| - | + | Crystal structure of the UBR-box from UBR6/FBXO11 reveals domain swapping mediated by zinc binding.,Munoz-Escobar J, Kozlov G, Gehring K Protein Sci. 2017 Jul 10. doi: 10.1002/pro.3227. PMID:28691247<ref>PMID:28691247</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 5vmd" style="background-color:#fffaf0;"></div> | |
| - | + | == References == | |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Gehring, K]] | [[Category: Gehring, K]] | ||
| + | [[Category: Kozlov, G]] | ||
| + | [[Category: Munoz-Escobar, J]] | ||
| + | [[Category: Domain swapping]] | ||
| + | [[Category: Fbxo11]] | ||
| + | [[Category: Ligase]] | ||
| + | [[Category: Ubr-box]] | ||
| + | [[Category: Ubr6]] | ||
| + | [[Category: Zinc]] | ||
| + | [[Category: Zinc finger]] | ||
Revision as of 09:10, 9 August 2017
Crystal structure of UBR-box from UBR6 in a domain-swapping conformation
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Categories: Gehring, K | Kozlov, G | Munoz-Escobar, J | Domain swapping | Fbxo11 | Ligase | Ubr-box | Ubr6 | Zinc | Zinc finger
