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5mzw
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of the decarboxylase AibA/AibB== | |
| + | <StructureSection load='5mzw' size='340' side='right' caption='[[5mzw]], [[Resolution|resolution]] 1.52Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5mzw]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MZW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5MZW FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5mzw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mzw OCA], [http://pdbe.org/5mzw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5mzw RCSB], [http://www.ebi.ac.uk/pdbsum/5mzw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5mzw ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Isovaleryl coenzyme A (IV-CoA) is an important precursor for iso-fatty acids and lipids. It acts in the development of myxobacteria, which can produce this compound from acetyl-CoA through alternative IV-CoA biosynthesis (aib). A central reaction of aib is catalyzed by AibA/AibB, which acts as a cofactor-free decarboxylase despite belonging to the family of CoA-transferases. We developed an efficient expression system for AibA/AibB that allowed the determination of high-resolution crystal structures in complex with different ligands. Through mutational studies, we show that an active-site cysteine previously proposed to be involved in decarboxylation is not required for activity. Instead, AibA/AibB seems to induce an intramolecular decarboxylation by binding its substrate in a hydrophobic cavity and forcing it into a bent conformation. Our study opens opportunities for synthetic biology studies, since AibA/AibB may be suitable for the production of isobutene, a precursor of biofuels and chemicals. | ||
| - | + | AibA/AibB Induces an Intramolecular Decarboxylation in Isovalerate Biosynthesis by Myxococcus xanthus.,Bock T, Luxenburger E, Hoffmann J, Schutza V, Feiler C, Muller R, Blankenfeldt W Angew Chem Int Ed Engl. 2017 Aug 7;56(33):9986-9989. doi: 10.1002/anie.201701992., Epub 2017 May 16. PMID:28508504<ref>PMID:28508504</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 5mzw" style="background-color:#fffaf0;"></div> | |
| - | + | == References == | |
| - | + | <references/> | |
| - | + | __TOC__ | |
| + | </StructureSection> | ||
[[Category: Blankenfeldt, W]] | [[Category: Blankenfeldt, W]] | ||
| + | [[Category: Bock, T]] | ||
[[Category: Feiler, C]] | [[Category: Feiler, C]] | ||
[[Category: Hoffmann, J]] | [[Category: Hoffmann, J]] | ||
| + | [[Category: Luxenburger, E]] | ||
| + | [[Category: Mueller, R]] | ||
| + | [[Category: Schuetza, V]] | ||
| + | [[Category: Coa transferase like fold]] | ||
| + | [[Category: Decarboxylase]] | ||
| + | [[Category: Lyase]] | ||
Revision as of 09:12, 9 August 2017
Crystal structure of the decarboxylase AibA/AibB
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