1w4r

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|PDB= 1w4r |SIZE=350|CAPTION= <scene name='initialview01'>1w4r</scene>, resolution 1.83&Aring;
|PDB= 1w4r |SIZE=350|CAPTION= <scene name='initialview01'>1w4r</scene>, resolution 1.83&Aring;
|SITE= <scene name='pdbsite=AC1:Dtu+Binding+Site+For+Chain+E'>AC1</scene>
|SITE= <scene name='pdbsite=AC1:Dtu+Binding+Site+For+Chain+E'>AC1</scene>
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|LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>, <scene name='pdbligand=TTP:THYMIDINE-5&#39;-TRIPHOSPHATE'>TTP</scene> and <scene name='pdbligand=DTU:(2R,3S)-1,4-DIMERCAPTOBUTANE-2,3-DIOL'>DTU</scene>
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|LIGAND= <scene name='pdbligand=DTU:(2R,3S)-1,4-DIMERCAPTOBUTANE-2,3-DIOL'>DTU</scene>, <scene name='pdbligand=TTP:THYMIDINE-5&#39;-TRIPHOSPHATE'>TTP</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Thymidine_kinase Thymidine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.21 2.7.1.21]
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Thymidine_kinase Thymidine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.21 2.7.1.21] </span>
|GENE=
|GENE=
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|DOMAIN=
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1w4r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1w4r OCA], [http://www.ebi.ac.uk/pdbsum/1w4r PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1w4r RCSB]</span>
}}
}}
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[[Category: Scapozza, L.]]
[[Category: Scapozza, L.]]
[[Category: Schulz, G E.]]
[[Category: Schulz, G E.]]
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[[Category: DTU]]
 
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[[Category: TTP]]
 
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[[Category: ZN]]
 
[[Category: cytosolic]]
[[Category: cytosolic]]
[[Category: human]]
[[Category: human]]
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[[Category: type ii]]
[[Category: type ii]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 23 14:04:02 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:31:09 2008''

Revision as of 21:31, 30 March 2008


PDB ID 1w4r

Drag the structure with the mouse to rotate
, resolution 1.83Å
Sites:
Ligands: , ,
Activity: Thymidine kinase, with EC number 2.7.1.21
Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



STRUCTURE OF A TYPE II THYMIDINE KINASE WITH BOUND DTTP


Overview

The structure of human cytosolic thymidine kinase in complex with its feedback inhibitor 2'-deoxythymidine-5'-triphosphate was determined. This structure is the first representative of the type II thymidine kinases found in several pathogens. The structure deviates strongly from the known structures of type I thymidine kinases such as the Herpes simplex enzyme. It contains a zinc-binding domain with four cysteines complexing a structural zinc ion. Interestingly, the backbone atoms of the type II enzyme bind thymine via hydrogen-bonds, in contrast to type I, where side chains are involved. This results in a specificity difference exploited for antiviral therapy. The presented structure will foster the development of new drugs and prodrugs for numerous therapeutic applications.

About this Structure

1W4R is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Structure of a type II thymidine kinase with bound dTTP., Birringer MS, Claus MT, Folkers G, Kloer DP, Schulz GE, Scapozza L, FEBS Lett. 2005 Feb 28;579(6):1376-82. PMID:15733844

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