1w7z

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|PDB= 1w7z |SIZE=350|CAPTION= <scene name='initialview01'>1w7z</scene>, resolution 1.67&Aring;
|PDB= 1w7z |SIZE=350|CAPTION= <scene name='initialview01'>1w7z</scene>, resolution 1.67&Aring;
|SITE= <scene name='pdbsite=AC1:Fmt+Binding+Site+For+Chain+F'>AC1</scene>
|SITE= <scene name='pdbsite=AC1:Fmt+Binding+Site+For+Chain+F'>AC1</scene>
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|LIGAND= <scene name='pdbligand=NA:SODIUM+ION'>NA</scene> and <scene name='pdbligand=FMT:FORMIC ACID'>FMT</scene>
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|LIGAND= <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>
|ACTIVITY=
|ACTIVITY=
|GENE=
|GENE=
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|DOMAIN=
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1w7z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1w7z OCA], [http://www.ebi.ac.uk/pdbsum/1w7z PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1w7z RCSB]</span>
}}
}}
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[[Category: Schneider, T R.]]
[[Category: Schneider, T R.]]
[[Category: Uson, I.]]
[[Category: Uson, I.]]
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[[Category: FMT]]
 
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[[Category: NA]]
 
[[Category: cystein knot]]
[[Category: cystein knot]]
[[Category: ecballium elaterium]]
[[Category: ecballium elaterium]]
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[[Category: trypsin]]
[[Category: trypsin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:53:24 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:32:29 2008''

Revision as of 21:32, 30 March 2008


PDB ID 1w7z

Drag the structure with the mouse to rotate
, resolution 1.67Å
Sites:
Ligands: ,
Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



CRYSTAL STRUCTURE OF THE FREE (UNCOMPLEXED) ECBALLIUM ELATERIUM TRYPSIN INHIBITOR (EETI-II)


Overview

The Ecballium elaterium trypsin inhibitor II (EETI-II) belongs to the family of squash inhibitors and is one of the strongest inhibitors known for trypsin. The eight independent molecules of EETI-II in the crystal structure reported here provide a good opportunity to test the hypothesis that this small cystine-knot protein (knottin) is sufficiently rigid to be used as a molecular scaffold for protein-engineering purposes. To extend this test, the structures of two complexes of EETI-II with trypsin have also been determined, one carrying a four-amino-acid mutation of EETI-II. The remarkable similarity of these structures confirms the rigidity of the molecular framework and hence its suitability as a molecular scaffold.

About this Structure

1W7Z is a Single protein structure of sequence from Ecballium elaterium. Full crystallographic information is available from OCA.

Reference

Structure of Ecballium elaterium trypsin inhibitor II (EETI-II): a rigid molecular scaffold., Kratzner R, Debreczeni JE, Pape T, Schneider TR, Wentzel A, Kolmar H, Sheldrick GM, Uson I, Acta Crystallogr D Biol Crystallogr. 2005 Sep;61(Pt 9):1255-62. Epub 2005, Aug 16. PMID:16131759

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