1w88

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|PDB= 1w88 |SIZE=350|CAPTION= <scene name='initialview01'>1w88</scene>, resolution 2.30&Aring;
|PDB= 1w88 |SIZE=350|CAPTION= <scene name='initialview01'>1w88</scene>, resolution 2.30&Aring;
|SITE= <scene name='pdbsite=AC1:Mg+Binding+Site+For+Chain+G'>AC1</scene>
|SITE= <scene name='pdbsite=AC1:Mg+Binding+Site+For+Chain+G'>AC1</scene>
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|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> and <scene name='pdbligand=TDP:THIAMIN DIPHOSPHATE'>TDP</scene>
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|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TDP:THIAMIN+DIPHOSPHATE'>TDP</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Pyruvate_dehydrogenase_(acetyl-transferring) Pyruvate dehydrogenase (acetyl-transferring)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.4.1 1.2.4.1]
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Pyruvate_dehydrogenase_(acetyl-transferring) Pyruvate dehydrogenase (acetyl-transferring)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.4.1 1.2.4.1] </span>
|GENE=
|GENE=
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|DOMAIN=
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1w88 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1w88 OCA], [http://www.ebi.ac.uk/pdbsum/1w88 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1w88 RCSB]</span>
}}
}}
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[[Category: Perham, R N.]]
[[Category: Perham, R N.]]
[[Category: Pratap, J V.]]
[[Category: Pratap, J V.]]
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[[Category: MG]]
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[[Category: catalysis,slinky]]
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[[Category: TDP]]
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[[Category: dihydrolipoyl,acetyl transferase]]
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[[Category: acetyl transferase]]
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[[Category: catalysis]]
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[[Category: dihydrolipoyl]]
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[[Category: multienzyme complex]]
[[Category: multienzyme complex]]
[[Category: oxidoreductase]]
[[Category: oxidoreductase]]
[[Category: pyruvate dehydrogenase]]
[[Category: pyruvate dehydrogenase]]
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[[Category: slinky]]
 
[[Category: transferase]]
[[Category: transferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:53:29 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:32:34 2008''

Revision as of 21:32, 30 March 2008

Image:1w88.gif


PDB ID 1w88

Drag the structure with the mouse to rotate
, resolution 2.30Å
Sites:
Ligands: ,
Activity: Pyruvate dehydrogenase (acetyl-transferring), with EC number 1.2.4.1
Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



THE CRYSTAL STRUCTURE OF PYRUVATE DEHYDROGENASE E1(D180N, E183Q) BOUND TO THE PERIPHERAL SUBUNIT BINDING DOMAIN OF E2


Overview

Thiamine diphosphate (ThDP) is used as a cofactor in many key metabolic enzymes. We present evidence that the ThDPs in the two active sites of the E1 (EC 1.2.4.1) component of the pyruvate dehydrogenase complex communicate over a distance of 20 angstroms by reversibly shuttling a proton through an acidic tunnel in the protein. This "proton wire" permits the co-factors to serve reciprocally as general acid/base in catalysis and to switch the conformation of crucial active-site peptide loops. This synchronizes the progression of chemical events and can account for the oligomeric organization, conformational asymmetry, and "ping-pong" kinetic properties of E1 and other thiamine-dependent enzymes.

About this Structure

1W88 is a Protein complex structure of sequences from Geobacillus stearothermophilus. Full crystallographic information is available from OCA.

Reference

A molecular switch and proton wire synchronize the active sites in thiamine enzymes., Frank RA, Titman CM, Pratap JV, Luisi BF, Perham RN, Science. 2004 Oct 29;306(5697):872-6. PMID:15514159

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