1wac
From Proteopedia
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|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1wac FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wac OCA], [http://www.ebi.ac.uk/pdbsum/1wac PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1wac RCSB]</span> | ||
}} | }} | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1WAC is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/ | + | 1WAC is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_phage_phi6 Pseudomonas phage phi6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WAC OCA]. |
==Reference== | ==Reference== | ||
Back-priming mode of phi6 RNA-dependent RNA polymerase., Laurila MR, Salgado PS, Stuart DI, Grimes JM, Bamford DH, J Gen Virol. 2005 Feb;86(Pt 2):521-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15659773 15659773] | Back-priming mode of phi6 RNA-dependent RNA polymerase., Laurila MR, Salgado PS, Stuart DI, Grimes JM, Bamford DH, J Gen Virol. 2005 Feb;86(Pt 2):521-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15659773 15659773] | ||
| - | [[Category: | + | [[Category: Pseudomonas phage phi6]] |
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Bamford, D H.]] | [[Category: Bamford, D H.]] | ||
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[[Category: transcription]] | [[Category: transcription]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:33:28 2008'' |
Revision as of 21:33, 30 March 2008
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| , resolution 3.0Å | |||||||
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
BACK-PRIMING MODE OF PHI6 RNA-DEPENDENT RNA POLYMERASE
Overview
The RNA-dependent RNA polymerase of the double-stranded RNA bacteriophage phi6 is capable of primer-independent initiation, as are many RNA polymerases. The structure of this polymerase revealed an initiation platform, composed of a loop in the C-terminal domain (QYKW, aa 629-632), that was essential for de novo initiation. A similar element has been identified in hepatitis C virus RNA-dependent RNA polymerase. Biochemical studies have addressed the role of this platform, revealing that a mutant version can utilize a back-priming initiation mechanism, where the 3' terminus of the template adopts a hairpin-like conformation. Here, the mechanism of back-primed initiation is studied further by biochemical and structural methods.
About this Structure
1WAC is a Single protein structure of sequence from Pseudomonas phage phi6. Full crystallographic information is available from OCA.
Reference
Back-priming mode of phi6 RNA-dependent RNA polymerase., Laurila MR, Salgado PS, Stuart DI, Grimes JM, Bamford DH, J Gen Virol. 2005 Feb;86(Pt 2):521-6. PMID:15659773
Page seeded by OCA on Mon Mar 31 00:33:28 2008
