5ndc

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'''Unreleased structure'''
 
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The entry 5ndc is ON HOLD until Paper Publication
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==Structure of ba3-type cytochrome c oxidase from Thermus thermophilus by serial femtosecond crystallography==
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<StructureSection load='5ndc' size='340' side='right' caption='[[5ndc]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[5ndc]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NDC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5NDC FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=CUA:DINUCLEAR+COPPER+ION'>CUA</scene>, <scene name='pdbligand=HAS:HEME-AS'>HAS</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=OLC:(2R)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLC</scene></td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cytochrome-c_oxidase Cytochrome-c oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.9.3.1 1.9.3.1] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ndc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ndc OCA], [http://pdbe.org/5ndc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ndc RCSB], [http://www.ebi.ac.uk/pdbsum/5ndc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ndc ProSAT]</span></td></tr>
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</table>
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== Function ==
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[[http://www.uniprot.org/uniprot/COX2_THET8 COX2_THET8]] Subunits I and II form the functional core of the enzyme complex. Electrons originating in cytochrome c are transferred via heme a and Cu(A) to the binuclear center formed by heme a3 and Cu(B).
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Cytochrome c oxidase catalyses the reduction of molecular oxygen to water while the energy released in this process is used to pump protons across a biological membrane. Although an extremely well-studied biological system, the molecular mechanism of proton pumping by cytochrome c oxidase is still not understood. Here we report a method to produce large quantities of highly diffracting microcrystals of ba 3-type cytochrome c oxidase from Thermus thermophilus suitable for serial femtosecond crystallography. The room-temperature structure of cytochrome c oxidase is solved to 2.3 A resolution from data collected at an X-ray Free Electron Laser. We find overall agreement with earlier X-ray structures solved from diffraction data collected at cryogenic temperature. Previous structures solved from synchrotron radiation data, however, have shown conflicting results regarding the identity of the active-site ligand. Our room-temperature structure, which is free from the effects of radiation damage, reveals that a single-oxygen species in the form of a water molecule or hydroxide ion is bound in the active site. Structural differences between the ba 3-type and aa 3-type cytochrome c oxidases around the proton-loading site are also described.
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Authors:
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Serial femtosecond crystallography structure of cytochrome c oxidase at room temperature.,Andersson R, Safari C, Dods R, Nango E, Tanaka R, Yamashita A, Nakane T, Tono K, Joti Y, Bath P, Dunevall E, Bosman R, Nureki O, Iwata S, Neutze R, Branden G Sci Rep. 2017 Jul 3;7(1):4518. doi: 10.1038/s41598-017-04817-z. PMID:28674417<ref>PMID:28674417</ref>
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Description:
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Unreleased Structures]]
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</div>
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<div class="pdbe-citations 5ndc" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Cytochrome-c oxidase]]
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[[Category: Andersson, R]]
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[[Category: Bath, P]]
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[[Category: Bosman, E]]
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[[Category: Branden, G]]
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[[Category: Dods, R]]
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[[Category: Dunevall, E]]
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[[Category: Iwata, S]]
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[[Category: Joti, Y]]
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[[Category: Nakane, T]]
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[[Category: Nango, E]]
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[[Category: Neutze, R]]
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[[Category: Nureki, O]]
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[[Category: Safari, C]]
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[[Category: Tanaka, R]]
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[[Category: Tono, K]]
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[[Category: Cytochrome c oxidase]]
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[[Category: Oxidoreductase]]
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[[Category: Respiratory chain enzyme]]
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[[Category: Sfx]]

Revision as of 11:04, 24 August 2017

Structure of ba3-type cytochrome c oxidase from Thermus thermophilus by serial femtosecond crystallography

5ndc, resolution 2.30Å

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