Structural highlights
Publication Abstract from PubMed
In several Proteobacteria, LuxI-type enzymes catalyze the biosynthesis of acyl-homoserine lactones (AHL) signals using S-adenosyl-l-methionine and either cellular acyl carrier protein (ACP)-coupled fatty acids or CoA-aryl/acyl moieties as progenitors. Little is known about the molecular mechanism of signal biosynthesis, the basis for substrate specificity, or the rationale for donor specificity for any LuxI member. Here, we present several cocrystal structures of BjaI, a CoA-dependent LuxI homolog that represent views of enzyme complexes that exist along the reaction coordinate of signal synthesis. Complementary biophysical, structure-function, and kinetic analysis define the features that facilitate the unusual acyl conjugation with S-adenosylmethionine (SAM). We also identify the determinant that establishes specificity for the acyl donor and identify residues that are critical for acyl/aryl specificity. These results highlight how a prevalent scaffold has evolved to catalyze quorum signal synthesis and provide a framework for the design of small-molecule antagonists of quorum signaling.
Molecular basis for the substrate specificity of quorum signal synthases.,Dong SH, Frane ND, Christensen QH, Greenberg EP, Nagarajan R, Nair SK Proc Natl Acad Sci U S A. 2017 Aug 22;114(34):9092-9097. doi:, 10.1073/pnas.1705400114. Epub 2017 Aug 7. PMID:28784791[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Dong SH, Frane ND, Christensen QH, Greenberg EP, Nagarajan R, Nair SK. Molecular basis for the substrate specificity of quorum signal synthases. Proc Natl Acad Sci U S A. 2017 Aug 22;114(34):9092-9097. doi:, 10.1073/pnas.1705400114. Epub 2017 Aug 7. PMID:28784791 doi:http://dx.doi.org/10.1073/pnas.1705400114
