1wbf
From Proteopedia
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|PDB= 1wbf |SIZE=350|CAPTION= <scene name='initialview01'>1wbf</scene>, resolution 2.30Å | |PDB= 1wbf |SIZE=350|CAPTION= <scene name='initialview01'>1wbf</scene>, resolution 2.30Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1wbf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wbf OCA], [http://www.ebi.ac.uk/pdbsum/1wbf PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1wbf RCSB]</span> | ||
}} | }} | ||
| Line 25: | Line 28: | ||
[[Category: Srinivas, V R.]] | [[Category: Srinivas, V R.]] | ||
[[Category: Suguna, K.]] | [[Category: Suguna, K.]] | ||
| - | [[Category: CA]] | ||
| - | [[Category: MN]] | ||
| - | [[Category: NAG]] | ||
[[Category: blood group specificity]] | [[Category: blood group specificity]] | ||
[[Category: lectin (agglutinin)]] | [[Category: lectin (agglutinin)]] | ||
| Line 34: | Line 34: | ||
[[Category: saccharide free form]] | [[Category: saccharide free form]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:33:56 2008'' |
Revision as of 21:33, 30 March 2008
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| , resolution 2.30Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
WINGED BEAN LECTIN, SACCHARIDE FREE FORM
Overview
The crystal structure of the saccharide-free form of the basic form of winged-bean agglutinin (WBAI) has been solved by the molecular-replacement method and refined at 2.3 A resolution. The final R factor is 19.7% for all data in the resolution range 8.0-2.3 A. The asymmetric unit contains two half-dimers, each located on a crystallographic twofold axis. The structure of the saccharide-free form is compared with that of the complex of WBAI with methyl-alpha-D-galactoside. The complex is composed of two dimers in the asymmetric unit. The intersubunit interactions in the dimer are nearly identical in the two structures. The binding site of the saccharide-free structure contains three ordered water molecules at positions similar to those of the hydroxyl groups of the carbohydrate which are hydrogen bonded to the protein. Superposition of the saccharide-binding sites of the two structures shows that the major changes involve expulsion of these ordered water molecules and a shift of about 0.6 A of the main-chain atoms of the variable loop.
About this Structure
1WBF is a Single protein structure of sequence from Psophocarpus tetragonolobus. Full crystallographic information is available from OCA.
Reference
Structure of basic winged-bean lectin and a comparison with its saccharide-bound form., Manoj N, Srinivas VR, Suguna K, Acta Crystallogr D Biol Crystallogr. 1999 Apr;55(Pt 4):794-800. PMID:10089310
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