1wcm

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|PDB= 1wcm |SIZE=350|CAPTION= <scene name='initialview01'>1wcm</scene>, resolution 3.8&Aring;
|PDB= 1wcm |SIZE=350|CAPTION= <scene name='initialview01'>1wcm</scene>, resolution 3.8&Aring;
|SITE= <scene name='pdbsite=AC1:Mg+Binding+Site+For+Chain+A'>AC1</scene>
|SITE= <scene name='pdbsite=AC1:Mg+Binding+Site+For+Chain+A'>AC1</scene>
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|LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> and <scene name='pdbligand=MG:MAGNESIUM ION'>MG</scene>
+
|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6]
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] </span>
|GENE=
|GENE=
 +
|DOMAIN=
 +
|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1wcm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wcm OCA], [http://www.ebi.ac.uk/pdbsum/1wcm PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1wcm RCSB]</span>
}}
}}
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[[Category: Meinhart, A.]]
[[Category: Meinhart, A.]]
[[Category: Mitterweger, S.]]
[[Category: Mitterweger, S.]]
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[[Category: MG]]
 
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[[Category: ZN]]
 
[[Category: cellular rna polymerase]]
[[Category: cellular rna polymerase]]
[[Category: dna-binding]]
[[Category: dna-binding]]
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[[Category: zinc-finger]]
[[Category: zinc-finger]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:55:16 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:34:25 2008''

Revision as of 21:34, 30 March 2008

Image:1wcm.gif


PDB ID 1wcm

Drag the structure with the mouse to rotate
, resolution 3.8Å
Sites:
Ligands: ,
Activity: DNA-directed RNA polymerase, with EC number 2.7.7.6
Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



COMPLETE 12-SUBUNIT RNA POLYMERASE II AT 3.8 ANG


Overview

We determined the x-ray structure of the RNA polymerase (Pol) II subcomplex Rpb4/7 at 2.3 A resolution, combined it with a previous structure of the 10-subunit polymerase core, and refined an atomic model of the complete 12-subunit Pol II at 3.8-A resolution. Comparison of the complete Pol II structure with structures of the Pol II core and free Rpb4/7 shows that the core-Rpb4/7 interaction goes along with formation of an alpha-helix in the linker region of the largest Pol II subunit and with folding of the conserved Rpb7 tip loop. Details of the core-Rpb4/7 interface explain facilitated Rpb4/7 dissociation in a temperature-sensitive Pol II mutant and specific assembly of Pol I with its Rpb4/7 counterpart, A43/14. The refined atomic model of Pol II serves as the new reference structure for analysis of the transcription mechanism and enables structure solution of complexes of the complete enzyme with additional factors and nucleic acids by molecular replacement.

About this Structure

1WCM is a Protein complex structure of sequences from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

Reference

Structures of complete RNA polymerase II and its subcomplex, Rpb4/7., Armache KJ, Mitterweger S, Meinhart A, Cramer P, J Biol Chem. 2005 Feb 25;280(8):7131-4. Epub 2004 Dec 9. PMID:15591044

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