5nbw
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of the Fab fragment 22F12 in complex with 3-hydroxybenzo[a]pyrene== | |
| + | <StructureSection load='5nbw' size='340' side='right' caption='[[5nbw]], [[Resolution|resolution]] 2.40Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5nbw]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NBW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5NBW FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=8SK:benzo[a]pyren-3-ol'>8SK</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5nbw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nbw OCA], [http://pdbe.org/5nbw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5nbw RCSB], [http://www.ebi.ac.uk/pdbsum/5nbw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5nbw ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Benzo[a]pyrene, which is produced during the incomplete combustion of organic material, is an abundant noxious pollutant because of its carcinogenic metabolic degradation products. The high-affinity (KD approximately 3 nm) monoclonal antibody 22F12 allows facile bioanalytical quantification of benzo[a]pyrene even in complex matrices. We report the functional and X-ray crystallographic analysis of 22F12 in complex with 3-hydroxybenzo[a]pyrene after cloning of the V-genes and production as a recombinant Fab fragment. The polycyclic aromatic hydrocarbon is bound in a deep pocket between the light and heavy chains, surrounded mainly by aromatic and aliphatic amino acid side chains. Interestingly, the hapten-antibody interface is less densely packed than expected and reveals polar, H-bond-like interactions with the polycyclic aromatic pi-electron system, which may allow the antibody to maintain a large, predominantly hydrophobic binding site in an aqueous environment while providing sufficient complementarity to its ligand. | ||
| - | + | Tight Molecular Recognition of Benzo[a]pyrene by a High-Affinity Antibody.,Eichinger A, Neumaier I, Pschenitza M, Niessner R, Knopp D, Skerra A Angew Chem Int Ed Engl. 2017 Aug 21;56(35):10592-10597. doi:, 10.1002/anie.201703893. Epub 2017 Jul 21. PMID:28603847<ref>PMID:28603847</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| + | <div class="pdbe-citations 5nbw" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Eichinger, A]] | [[Category: Eichinger, A]] | ||
[[Category: Skerra, A]] | [[Category: Skerra, A]] | ||
| + | [[Category: Antibody]] | ||
| + | [[Category: Fab fragment]] | ||
| + | [[Category: Hapten]] | ||
| + | [[Category: Immune system]] | ||
Revision as of 04:16, 30 August 2017
Crystal structure of the Fab fragment 22F12 in complex with 3-hydroxybenzo[a]pyrene
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