5ei0

Publication Abstract from PubMed

The adipokine vaspin (serpinA12) is mainly expressed in white adipose tissue and exhibits various beneficial effects on obesity-related processes. Kallikrein 7 is the only known target protease of vaspin and is inhibited by the classical serpin inhibitory mechanism involving a cleavage of the reactive center loop between P1 (M378) and P1' (E379). Here, we present the x-ray structure of vaspin, cleaved between M378-E379. We provide a comprehensive analysis of differences between the uncleaved and cleaved forms in the shutter, breach, and hinge regions as well as helix F with relation to common molecular features underlying the serpin inhibitory mode. Furthermore, we point out differences towards other serpins and provide novel data underlining the remarkable stability of vaspin. We speculate that the previously reported FKGx1Wx2x3 motif in the breach region may play a decisive role in determining the reactive center loop configuration in the native vaspin state and might contribute to the high thermostability of vaspin. Thus, this structure may provide a basis for future mutational studies.

Crystal structure of cleaved vaspin (serpinA12).,Pippel J, Kuettner EB, Ulbricht D, Daberger J, Schultz S, Heiker JT, Strater N Biol Chem. 2015 Nov 3. pii:, /j/bchm.just-accepted/hsz-2015-0229/hsz-2015-0229.xml. doi:, 10.1515/hsz-2015-0229. PMID:26529565^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.