5m2g
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==PCE reductive dehalogenase from S. multivorans in complex with 2,4,6-tribromophenol== | |
| + | <StructureSection load='5m2g' size='340' side='right' caption='[[5m2g]], [[Resolution|resolution]] 1.80Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5m2g]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Sulfurospirillum_multivorans Sulfurospirillum multivorans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5M2G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5M2G FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BEN:BENZAMIDINE'>BEN</scene>, <scene name='pdbligand=BVQ:NORPSEUDO-B12'>BVQ</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene>, <scene name='pdbligand=TBP:2,4,6-TRIBROMOPHENOL'>TBP</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5m2g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5m2g OCA], [http://pdbe.org/5m2g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5m2g RCSB], [http://www.ebi.ac.uk/pdbsum/5m2g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5m2g ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The capacity of metal-containing porphyrinoids to mediate reductive dehalogenation is implemented in cobamide-containing reductive dehalogenases (RDases), which serve as terminal reductases in organohalide-respiring microbes. RDases allow for the exploitation of halogenated compounds as electron acceptors. Their reaction mechanism is under debate. Here we report on substrate-enzyme interactions in a tetrachloroethene RDase (PceA) that also converts aryl halides. The shape of PceA's highly apolar active site directs binding of bromophenols at some distance from the cobalt and with the hydroxyl substituent towards the metal. A close cobalt-substrate interaction is not observed by electron paramagnetic resonance spectroscopy. Nonetheless, a halogen substituent para to the hydroxyl group is reductively eliminated and the path of the leaving halide is traced in the structure. Based on these findings, an enzymatic mechanism relying on a long-range electron transfer is concluded, which is without parallel in vitamin B12-dependent biochemistry and represents an effective mode of RDase catalysis. | ||
| - | + | Cobamide-mediated enzymatic reductive dehalogenation via long-range electron transfer.,Kunze C, Bommer M, Hagen WR, Uksa M, Dobbek H, Schubert T, Diekert G Nat Commun. 2017 Jul 3;8:15858. doi: 10.1038/ncomms15858. PMID:28671181<ref>PMID:28671181</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 5m2g" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Sulfurospirillum multivorans]] | ||
| + | [[Category: Bommer, M]] | ||
| + | [[Category: Diekert, G]] | ||
[[Category: Dobbek, H]] | [[Category: Dobbek, H]] | ||
| + | [[Category: Hagen, W R]] | ||
[[Category: Kunze, C]] | [[Category: Kunze, C]] | ||
[[Category: Schubert, T]] | [[Category: Schubert, T]] | ||
| - | [[Category: Hagen, W.R]] | ||
| - | [[Category: Bommer, M]] | ||
[[Category: Uksa, M]] | [[Category: Uksa, M]] | ||
| - | [[Category: | + | [[Category: Organohalide respiration anaerobic crystallisation cobalamin]] |
| + | [[Category: Oxidoreductase]] | ||
Revision as of 10:25, 10 September 2017
PCE reductive dehalogenase from S. multivorans in complex with 2,4,6-tribromophenol
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