1wmw

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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1wmw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wmw OCA], [http://www.ebi.ac.uk/pdbsum/1wmw PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1wmw RCSB]</span>
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[[Category: thermus]]
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Revision as of 21:38, 30 March 2008

Image:1wmw.gif


PDB ID 1wmw

Drag the structure with the mouse to rotate
, resolution 1.55Å
Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



Crystal structure of geranulgeranyl diphosphate synthase from Thermus thermophilus


Overview

Geranylgeranyl diphosphate (GGPP) synthase from Thermus thermophilus HB8 was expressed in Escherichia coli, purified to homogeneity and crystallized both as the recombinant native protein and its selenomethionine (SeMet) derivative. Well diffracting crystals of these proteins were obtained belonging to the tetragonal space group P4(1) or P4(3), with unit-cell parameters a = b = 139.88, c = 73.37 A. There were two homodimers in the asymmetric unit. A native data set was collected to 1.55 A resolution and a data set suitable for MAD phasing was collected to 2.40 A resolution on beamline BL40B2 at SPring-8.

About this Structure

1WMW is a Single protein structure of sequence from Thermus thermophilus. Full crystallographic information is available from OCA.

Reference

Expression, purification, crystallization and preliminary X-ray studies of geranylgeranyl diphosphate synthase from Thermus thermophilus HB8., Nishio K, Nodake Y, Hamada K, Suto K, Nakagawa N, Kuramitsu S, Miura K, Acta Crystallogr D Biol Crystallogr. 2004 Jan;60(Pt 1):178-80. Epub 2003, Dec 18. PMID:14684922

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