Structural highlights
Function
[CONA_CANEN] D-mannose specific lectin.
Publication Abstract from PubMed
Plant lectins are carbohydrate-binding proteins with various biomedical applications. Concanavalin A (Con A) holds promise in treating cancerous tumors. To better understand the Con A carbohydrate binding specificity, we obtained a room-temperature neutron structure of this legume lectin in complex with a disaccharide Manalpha1-2Man, mannobiose. The neutron structure afforded direct visualization of the hydrogen bonding between the protein and ligand, showing that the ligand is able to alter both protonation states and interactions for residues located close to and distant from the binding site. An unprecedented low-barrier hydrogen bond was observed forming between the carboxylic side chains of Asp28 and Glu8, with the D atom positioned equidistant from the oxygen atoms having an O...D...O angle of 101.5 degrees .
Mannobiose Binding Induces Changes in Hydrogen Bonding and Protonation States of Acidic Residues in Concanavalin A As Revealed by Neutron Crystallography.,Gerlits OO, Coates L, Woods RJ, Kovalevsky A Biochemistry. 2017 Sep 12;56(36):4747-4750. doi: 10.1021/acs.biochem.7b00654., Epub 2017 Aug 30. PMID:28846383[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Gerlits OO, Coates L, Woods RJ, Kovalevsky A. Mannobiose Binding Induces Changes in Hydrogen Bonding and Protonation States of Acidic Residues in Concanavalin A As Revealed by Neutron Crystallography. Biochemistry. 2017 Sep 12;56(36):4747-4750. doi: 10.1021/acs.biochem.7b00654., Epub 2017 Aug 30. PMID:28846383 doi:http://dx.doi.org/10.1021/acs.biochem.7b00654
