5unk
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==NMR structure of the RED subdomain of the Sleeping Beauty transposase== | |
| + | <StructureSection load='5unk' size='340' side='right' caption='[[5unk]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5unk]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UNK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5UNK FirstGlance]. <br> | ||
| + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5unk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5unk OCA], [http://pdbe.org/5unk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5unk RCSB], [http://www.ebi.ac.uk/pdbsum/5unk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5unk ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | DNA transposons can be employed for stable gene transfer in vertebrates. The Sleeping Beauty (SB) DNA transposon has been recently adapted for human application and is being evaluated in clinical trials, however its molecular mechanism is not clear. SB transposition is catalyzed by the transposase enzyme, which is a multi-domain protein containing the catalytic and the DNA-binding domains. The DNA-binding domain of the SB transposase contains two structurally independent subdomains, PAI and RED. Recently, the structures of the catalytic domain and the PAI subdomain have been determined, however no structural information on the RED subdomain and its interactions with DNA has been available. Here, we used NMR spectroscopy to determine the solution structure of the RED subdomain and characterize its interactions with the transposon DNA. | ||
| - | + | NMR solution structure of the RED subdomain of the Sleeping Beauty transposase.,Konnova TA, Singer CM, Nesmelova IV Protein Sci. 2017 Jun;26(6):1171-1181. doi: 10.1002/pro.3167. Epub 2017 Apr 2. PMID:28345263<ref>PMID:28345263</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: Nesmelova, I | + | <div class="pdbe-citations 5unk" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | == References == |
| - | [[Category: | + | <references/> |
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Konnova, T A]] | ||
| + | [[Category: Nesmelova, I V]] | ||
| + | [[Category: Singer, C M]] | ||
| + | [[Category: Dna binding protein]] | ||
| + | [[Category: Dna-binding]] | ||
| + | [[Category: Helical]] | ||
| + | [[Category: Helix-turn-helix]] | ||
| + | [[Category: Transposase]] | ||
Revision as of 10:40, 13 September 2017
NMR structure of the RED subdomain of the Sleeping Beauty transposase
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