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5fmv

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Revision as of 10:49, 13 September 2017

Crystal structure of human CD45 extracellular region, domains d1-d4

Structural highlights

5fmv is a 2 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Related:	5fn6, 5fn7, 5fn8
Activity:	Protein-tyrosine-phosphatase, with EC number 3.1.3.48
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Disease

[PTPRC_HUMAN] T-B+ severe combined immunodeficiency due to CD45 deficiency. The disease is caused by mutations affecting the gene represented in this entry. Disease susceptibility may be associated with variations affecting the gene represented in this entry.

Function

[PTPRC_HUMAN] Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity).

Publication Abstract from PubMed

It has been proposed that the local segregation of kinases and the tyrosine phosphatase CD45 underpins T cell antigen receptor (TCR) triggering, but how such segregation occurs and whether it can initiate signaling is unclear. Using structural and biophysical analysis, we show that the extracellular region of CD45 is rigid and extends beyond the distance spanned by TCR-ligand complexes, implying that sites of TCR-ligand engagement would sterically exclude CD45. We also show that the formation of 'close contacts', new structures characterized by spontaneous CD45 and kinase segregation at the submicron-scale, initiates signaling even when TCR ligands are absent. Our work reveals the structural basis for, and the potent signaling effects of, local CD45 and kinase segregation. TCR ligands have the potential to heighten signaling simply by holding receptors in close contacts.

Initiation of T cell signaling by CD45 segregation at 'close contacts'.,Chang VT, Fernandes RA, Ganzinger KA, Lee SF, Siebold C, McColl J, Jonsson P, Palayret M, Harlos K, Coles CH, Jones EY, Lui Y, Huang E, Gilbert RJ, Klenerman D, Aricescu AR, Davis SJ Nat Immunol. 2016 May;17(5):574-82. doi: 10.1038/ni.3392. Epub 2016 Mar 21. PMID:26998761^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Chang VT, Fernandes RA, Ganzinger KA, Lee SF, Siebold C, McColl J, Jonsson P, Palayret M, Harlos K, Coles CH, Jones EY, Lui Y, Huang E, Gilbert RJ, Klenerman D, Aricescu AR, Davis SJ. Initiation of T cell signaling by CD45 segregation at 'close contacts'. Nat Immunol. 2016 May;17(5):574-82. doi: 10.1038/ni.3392. Epub 2016 Mar 21. PMID:26998761 doi:http://dx.doi.org/10.1038/ni.3392

1 Structural highlights
2 Disease
3 Function
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5fmv"

@@ Line 7: / Line 7: @@
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5fn6|5fn6]], [[5fn7|5fn7]], [[5fn8|5fn8]]</td></tr>
 <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Protein-tyrosine-phosphatase Protein-tyrosine-phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.48 3.1.3.48] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5fmv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fmv OCA], [http://pdbe.org/5fmv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5fmv RCSB], [http://www.ebi.ac.uk/pdbsum/5fmv PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5fmv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fmv OCA], [http://pdbe.org/5fmv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5fmv RCSB], [http://www.ebi.ac.uk/pdbsum/5fmv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5fmv ProSAT]</span></td></tr>
 </table>
 == Disease ==
@@ Line 22: / Line 22: @@
 </div>
 <div class="pdbe-citations 5fmv" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Tyrosine phosphatase|Tyrosine phosphatase]]
 == References ==
 <references/>

5fmv

From Proteopedia

Revision as of 10:49, 13 September 2017

Crystal structure of human CD45 extracellular region, domains d1-d4

Structural highlights

Disease

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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