5t4w
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of a DNA sequence d (CGTGAATTCACG) with DAPI== | |
| + | <StructureSection load='5t4w' size='340' side='right' caption='[[5t4w]], [[Resolution|resolution]] 2.30Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5t4w]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5T4W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5T4W FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DAP:6-AMIDINE-2-(4-AMIDINO-PHENYL)INDOLE'>DAP</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5t4w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5t4w OCA], [http://pdbe.org/5t4w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5t4w RCSB], [http://www.ebi.ac.uk/pdbsum/5t4w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5t4w ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The structure of 4',6-diamidine-2-phenylindole (DAPI) bound to the synthetic B-DNA oligonucleotide d(CGTGAATTCACG) has been solved in space group P212121 by single-crystal X-ray diffraction at a resolution of 2.2 A. The structure is nearly isomorphous to that of the previously reported crystal structure of the oligonucleotide d(CGTGAATTCACG) alone. The adjustments in crystal packing between the native DNA molecule and the DNA-DAPI complex are described. DAPI lies in the narrow minor groove near the centre of the B-DNA fragment, positioned over the A-T base pairs. It is bound to the DNA by hydrogen-bonding and van der Waals interactions. Comparison of the two structures (with and without ligand) shows that DAPI inserts into the minor groove, displacing the ordered spine waters. Indeed, as DAPI is hydrophobic it confers this behaviour on the DNA and thus restricts the presence of water molecules. | ||
| - | + | Crystal structure of the DNA sequence d(CGTGAATTCACG)2 with DAPI.,Sbirkova-Dimitrova HI, Shivachev B Acta Crystallogr F Struct Biol Commun. 2017 Sep 1;73(Pt 9):500-504. doi:, 10.1107/S2053230X17011384. Epub 2017 Aug 14. PMID:28876227<ref>PMID:28876227</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | <div class="pdbe-citations 5t4w" style="background-color:#fffaf0;"></div> | |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Sbirkova, H]] | [[Category: Sbirkova, H]] | ||
| + | [[Category: Schivachev, B]] | ||
| + | [[Category: Dapi]] | ||
| + | [[Category: Dna]] | ||
| + | [[Category: Ligand]] | ||
Revision as of 04:37, 21 September 2017
Crystal structure of a DNA sequence d (CGTGAATTCACG) with DAPI
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Categories: Sbirkova, H | Schivachev, B | Dapi | Dna | Ligand
