This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1wu4
From Proteopedia
| Line 4: | Line 4: | ||
|PDB= 1wu4 |SIZE=350|CAPTION= <scene name='initialview01'>1wu4</scene>, resolution 1.35Å | |PDB= 1wu4 |SIZE=350|CAPTION= <scene name='initialview01'>1wu4</scene>, resolution 1.35Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Oligosaccharide_reducing-end_xylanase Oligosaccharide reducing-end xylanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.156 3.2.1.156] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Oligosaccharide_reducing-end_xylanase Oligosaccharide reducing-end xylanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.156 3.2.1.156] </span> |
|GENE= BH2105 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2 Bacteria]) | |GENE= BH2105 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2 Bacteria]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1wu5|1WU5]], [[1wu6|1WU6]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1wu4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wu4 OCA], [http://www.ebi.ac.uk/pdbsum/1wu4 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1wu4 RCSB]</span> | ||
}} | }} | ||
| Line 29: | Line 32: | ||
[[Category: Shoun, H.]] | [[Category: Shoun, H.]] | ||
[[Category: Wakagi, T.]] | [[Category: Wakagi, T.]] | ||
| - | [[Category: GOL]] | ||
| - | [[Category: NI]] | ||
[[Category: (alpla/alpha)6 barrel]] | [[Category: (alpla/alpha)6 barrel]] | ||
[[Category: glycoside hydrolase family 8]] | [[Category: glycoside hydrolase family 8]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:41:04 2008'' |
Revision as of 21:41, 30 March 2008
| |||||||
| , resolution 1.35Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||
| Gene: | BH2105 (Bacteria) | ||||||
| Activity: | Oligosaccharide reducing-end xylanase, with EC number 3.2.1.156 | ||||||
| Related: | 1WU5, 1WU6
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of reducing-end-xylose releasing exo-oligoxylanase
Overview
Reducing end xylose-releasing exo-oligoxylanase from Bacillus halodurans C-125 (Rex) hydrolyzes xylooligosaccharides whose degree of polymerization is greater than or equal to 3, releasing the xylose unit at the reducing end. It is a unique exo-type glycoside hydrolase that recognizes the xylose unit at the reducing end in a very strict manner, even discriminating the beta-anomeric hydroxyl configuration from the alpha-anomer or 1-deoxyxylose. We have determined the crystal structures of Rex in unliganded and complex forms at 1.35-2.20-A resolution and revealed the structural aspects of its three subsites ranging from -2 to +1. The structure of Rex was compared with those of endo-type enzymes in glycoside hydrolase subfamily 8a (GH-8a). The catalytic machinery of Rex is basically conserved with other GH-8a enzymes. However, subsite +2 is blocked by a barrier formed by a kink in the loop before helix alpha10. His-319 in this loop forms a direct hydrogen bond with the beta-hydroxyl of xylose at subsite +1, contributing to the specific recognition of anomers at the reducing end.
About this Structure
1WU4 is a Single protein structure of sequence from Bacteria. Full crystallographic information is available from OCA.
Reference
Structural basis for the specificity of the reducing end xylose-releasing exo-oligoxylanase from Bacillus halodurans C-125., Fushinobu S, Hidaka M, Honda Y, Wakagi T, Shoun H, Kitaoka M, J Biol Chem. 2005 Apr 29;280(17):17180-6. Epub 2005 Feb 17. PMID:15718242
Page seeded by OCA on Mon Mar 31 00:41:04 2008
