1wzz
From Proteopedia
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|PDB= 1wzz |SIZE=350|CAPTION= <scene name='initialview01'>1wzz</scene>, resolution 1.65Å | |PDB= 1wzz |SIZE=350|CAPTION= <scene name='initialview01'>1wzz</scene>, resolution 1.65Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene> | + | |LIGAND= <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] </span> |
|GENE= cmcAX ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=28448 Gluconacetobacter xylinus]) | |GENE= cmcAX ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=28448 Gluconacetobacter xylinus]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1wzz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wzz OCA], [http://www.ebi.ac.uk/pdbsum/1wzz PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1wzz RCSB]</span> | ||
}} | }} | ||
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[[Category: Yao, M.]] | [[Category: Yao, M.]] | ||
[[Category: Yasutake, Y.]] | [[Category: Yasutake, Y.]] | ||
| - | [[Category: SO4]] | ||
[[Category: (alpha/alpha)6 barrel]] | [[Category: (alpha/alpha)6 barrel]] | ||
[[Category: glycoside hydrolase family 8 (gh-8)]] | [[Category: glycoside hydrolase family 8 (gh-8)]] | ||
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[[Category: structural genomics consortium for research on gene expression system]] | [[Category: structural genomics consortium for research on gene expression system]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:43:09 2008'' |
Revision as of 21:43, 30 March 2008
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| , resolution 1.65Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||
| Gene: | cmcAX (Gluconacetobacter xylinus) | ||||||
| Activity: | Cellulase, with EC number 3.2.1.4 | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structure of endo-beta-1,4-glucanase CMCax from Acetobacter xylinum
Overview
Previous studies have demonstrated that endoglucanase is required for cellulose biosynthesis both in bacteria and plants. However, it has yet to be elucidated how the endoglucanases function in the mechanism of cellulose biosynthesis. Here we describe the crystal structure of the cellulose biosynthesis-related endo-beta-1,47-glucanase (CMCax; EC 3.2.1.4) from the cellulose-producing Gramnegative bacterium, Acetobacter xylinum (= Gluconacetobacter xylinus), determined at 1.65-A resolution. CMCax falls into the glycoside hydrolase family 8 (GH-8), and the structure showed that the overall fold of the CMCax is similar to those of other glycoside hydrolases belonging to GH-8. Structure comparison with Clostridium thermocellum CelA, the best characterized GH-8 endoglucanase, revealed that sugar recognition subsite +3 is completely missing in CMCax. The absence of the subsite +3 leads to significant broadness of the cleft at the cellooligosaccharide reducing-end side. CMCax is known to be a secreted enzyme and is present in the culture medium. However, electron microscopic analysis using immunostaining clearly demonstrated that a portion of CMCax is localized to the cell surface, suggesting a link with other known membrane-anchored endoglucanases that are required for cellulose biosynthesis.
About this Structure
1WZZ is a Single protein structure of sequence from Gluconacetobacter xylinus. Full crystallographic information is available from OCA.
Reference
Structural characterization of the Acetobacter xylinum endo-beta-1,4-glucanase CMCax required for cellulose biosynthesis., Yasutake Y, Kawano S, Tajima K, Yao M, Satoh Y, Munekata M, Tanaka I, Proteins. 2006 Sep 1;64(4):1069-77. PMID:16804941
Page seeded by OCA on Mon Mar 31 00:43:09 2008
Categories: Cellulase | Gluconacetobacter xylinus | Single protein | Kawano, S. | Munekata, M. | SGC, Structural Genomics Consortium. | Satoh, Y. | Tajima, K. | Tanaka, I. | Yao, M. | Yasutake, Y. | (alpha/alpha)6 barrel | Glycoside hydrolase family 8 (gh-8) | Sgcge | Structural genomic | Structural genomics consortium for research on gene expression system
