1ik3

From Proteopedia

Revision as of 14:34, 5 November 2007

LIPOXYGENASE-3 (SOYBEAN) COMPLEX WITH 13(S)-HYDROPEROXY-9(Z),11(E)-OCTADECADIENOIC ACID

Overview

Polyunsaturated fatty acid metabolism is governed primarily by two, enzymes, prostaglandin H synthase and lipoxygenase. The crystal structure, of the metastable product-oxidized purple form of soybean lipoxygenase-3, was determined at 2.0 A resolution. The data reveal that the chromophore, corresponds to an iron-peroxide complex, a potential intermediate in the, catalyzed reaction. A significant alteration of the iron site accompanies, the formation of the complex. The structure, the first for a fatty, acid-lipoxygenase complex, also reveals an unexpected mode of binding, and, identifies amino acid residues that may play significant roles in, catalysis, regio- and stereoselectivity.

About this Structure

1IK3 is a Single protein structure of sequence from Glycine max with FE, 13S, 13R, 9OH and 11O as ligands. Active as Lipoxygenase, with EC number 1.13.11.12 Structure known Active Site: FE3. Full crystallographic information is available from OCA.

Reference

Three-dimensional structure of a purple lipoxygenase., Skrzypczak-Jankun E, Bross RA, Carroll RT, Dunham WR, Funk MO Jr, J Am Chem Soc. 2001 Nov 7;123(44):10814-20. PMID:11686682

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