1x77

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|GENE=
|GENE=
|DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=COG0431 COG0431]</span>
|DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=COG0431 COG0431]</span>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1x77 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1x77 OCA], [http://www.ebi.ac.uk/pdbsum/1x77 PDBsum], [http://www.fli-leibniz.de/cgi-bin/ImgLib.pl?CODE=1kfv JenaLib], [http://www.rcsb.org/pdb/explore.do?structureId=1x77 RCSB]</span>
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|RELATEDENTRY=[[1rtt|1RTT]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1x77 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1x77 OCA], [http://www.ebi.ac.uk/pdbsum/1x77 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1x77 RCSB]</span>
}}
}}
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[[Category: structural genomic]]
[[Category: structural genomic]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Mar 26 06:10:34 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:45:39 2008''

Revision as of 21:45, 30 March 2008

Image:1x77.gif


PDB ID 1x77

Drag the structure with the mouse to rotate
, resolution 2.70Å
Ligands:
Domains: COG0431
Related: 1RTT


Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



Crystal struture of a NAD(P)H-dependent FMN reductase complexed with FMN


Overview

The availability of high-intensity synchrotron facilities, technological advances in data-collection techniques and improved data-reduction and crystallographic software have ushered in a new era in high-throughput macromolecular crystallography. Here, the de novo automated crystal structure determination at 1.28 A resolution of an NAD(P)H-dependent FMN reductase flavoprotein from Pseudomonas aeruginosa PA01-derived protein Q9I4D4 using the anomalous signal from an unusually small number of S atoms is reported. Although this protein lacks the flavodoxin key fingerprint motif [(T/S)XTGXT], it has been confirmed to bind flavin mononucleotide and the binding site was identified via X-ray crystallography. This protein contains a novel flavin mononucleotide-binding site GSLRSGSYN, which has not been previously reported. Detailed statistics pertaining to sulfur phasing and other factors contributing to structure determination are discussed. Structural comparisons of the apoenzyme and the protein complexed with flavin mononucleotide show conformational changes on cofactor binding. NADPH-dependent activity has been confirmed with biochemical assays.

About this Structure

1X77 is a Single protein structure of sequence from Pseudomonas aeruginosa pao1. Full crystallographic information is available from OCA.

Reference

Structure determination of an FMN reductase from Pseudomonas aeruginosa PA01 using sulfur anomalous signal., Agarwal R, Bonanno JB, Burley SK, Swaminathan S, Acta Crystallogr D Biol Crystallogr. 2006 Apr;62(Pt 4):383-91. Epub 2006, Mar 18. PMID:16552139

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