1xkg

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|PDB= 1xkg |SIZE=350|CAPTION= <scene name='initialview01'>1xkg</scene>, resolution 1.61&Aring;
|PDB= 1xkg |SIZE=350|CAPTION= <scene name='initialview01'>1xkg</scene>, resolution 1.61&Aring;
|SITE=
|SITE=
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|LIGAND= <scene name='pdbligand=YT3:YTTRIUM+(III)+ION'>YT3</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> and <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>
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|LIGAND= <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=YT3:YTTRIUM+(III)+ION'>YT3</scene>
|ACTIVITY=
|ACTIVITY=
|GENE= DERP1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6956 Dermatophagoides pteronyssinus])
|GENE= DERP1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6956 Dermatophagoides pteronyssinus])
 +
|DOMAIN=
 +
|RELATEDENTRY=
 +
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xkg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xkg OCA], [http://www.ebi.ac.uk/pdbsum/1xkg PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1xkg RCSB]</span>
}}
}}
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[[Category: Meno, K.]]
[[Category: Meno, K.]]
[[Category: Thorsted, P B.]]
[[Category: Thorsted, P B.]]
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[[Category: GOL]]
 
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[[Category: SO4]]
 
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[[Category: YT3]]
 
[[Category: cysteine protease]]
[[Category: cysteine protease]]
[[Category: dermatophagoides pteronyssinus]]
[[Category: dermatophagoides pteronyssinus]]
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[[Category: pro peptide]]
[[Category: pro peptide]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:10:58 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:50:51 2008''

Revision as of 21:50, 30 March 2008


PDB ID 1xkg

Drag the structure with the mouse to rotate
, resolution 1.61Å
Ligands: , ,
Gene: DERP1 (Dermatophagoides pteronyssinus)
Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



Crystal structure of the major house dust mite allergen Der p 1 in its pro form at 1.61 A resolution


Overview

Allergy to house dust mite is among the most prevalent allergic diseases worldwide. Most house dust mite allergic patients react to Der p 1 from Dermatophagoides pteronyssinus, which is a cysteine protease. To avoid heterogeneity in the sample used for crystallization, a modified recombinant molecule was produced. The sequence of the proDer p 1 allergen was modified to reduce glycosylation and to abolish enzymatic activity. The resulting rproDer p 1 preparation was homogenous and stable and yielded crystals diffracting to a resolution of 1.61 A. The active site is located in a large cleft on the surface of the molecule. The 80-aa pro-peptide adopts a unique fold that interacts with the active site cleft and a substantial adjacent area on the mature region, excluding access to the cleft and the active site. Studies performed using crossed-line immunoelectrophoresis and IgE inhibition experiments indicated that several epitopes are covered by the pro-peptide and that the epitopes on the recombinant mature molecule are indistinguishable from those on the natural one. The structure confirms previous results suggesting a preference for aliphatic residues in the important P2 position in substrates. Sequence variations in related species are concentrated on the surface, which explains the existence of cross-reacting and species-specific antibodies. This study describes the first crystal structure of one of the clinically most important house dust mite allergens, the cysteine protease Der p 1.

About this Structure

1XKG is a Single protein structure of sequence from Dermatophagoides pteronyssinus. Full crystallographic information is available from OCA.

Reference

The crystal structure of recombinant proDer p 1, a major house dust mite proteolytic allergen., Meno K, Thorsted PB, Ipsen H, Kristensen O, Larsen JN, Spangfort MD, Gajhede M, Lund K, J Immunol. 2005 Sep 15;175(6):3835-45. PMID:16148130

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