1xkw
From Proteopedia
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|PDB= 1xkw |SIZE=350|CAPTION= <scene name='initialview01'>1xkw</scene>, resolution 2.0Å | |PDB= 1xkw |SIZE=350|CAPTION= <scene name='initialview01'>1xkw</scene>, resolution 2.0Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=188:PYOCHELIN+FE(III)'>188</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=LDA:LAURYL+DIMETHYLAMINE-N-OXIDE'>LDA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xkw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xkw OCA], [http://www.ebi.ac.uk/pdbsum/1xkw PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1xkw RCSB]</span> | ||
}} | }} | ||
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[[Category: Cobessi, D.]] | [[Category: Cobessi, D.]] | ||
[[Category: Pattus, F.]] | [[Category: Pattus, F.]] | ||
| - | [[Category: 188]] | ||
| - | [[Category: EDO]] | ||
| - | [[Category: LDA]] | ||
| - | [[Category: SO4]] | ||
[[Category: tonb dependent receptor]] | [[Category: tonb dependent receptor]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:51:02 2008'' |
Revision as of 21:51, 30 March 2008
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| , resolution 2.0Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , , | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Pyochelin outer membrane receptor FptA from Pseudomonas aeruginosa
Overview
Pyochelin is a siderophore and virulence factor common to Burkholderia cepacia and several Pseudomonas strains. We describe at 2.0 A resolution the crystal structure of the pyochelin outer membrane receptor FptA bound to the iron-pyochelin isolated from Pseudomonas aeruginosa. One pyochelin molecule bound to iron is found in the protein structure, providing the first three-dimensional structure at the atomic level of this siderophore. The pyochelin molecule provides a tetra-dentate coordination of iron, while the remaining bi-dentate coordination is ensured by another molecule not specifically recognized by the protein. The overall structure of the pyochelin receptor is typical of the TonB-dependent transporter superfamily, which uses the proton motive force from the cytoplasmic membrane through the TonB-ExbB-ExbD energy transducing complex to transport ferric ions across the bacterial outer membrane: a transmembrane 22 beta-stranded barrel occluded by a N-terminal domain that contains a mixed four-stranded beta-sheet. The N-terminal TonB box is disordered in two crystal forms, and loop L8 is found to point towards the iron-pyochelin complex, suggesting that the receptor is in a transport-competent conformation.
About this Structure
1XKW is a Single protein structure of sequence from Pseudomonas aeruginosa. Full crystallographic information is available from OCA.
Reference
Crystal structure at high resolution of ferric-pyochelin and its membrane receptor FptA from Pseudomonas aeruginosa., Cobessi D, Celia H, Pattus F, J Mol Biol. 2005 Sep 30;352(4):893-904. PMID:16139844
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