1xxs
From Proteopedia
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|PDB= 1xxs |SIZE=350|CAPTION= <scene name='initialview01'>1xxs</scene>, resolution 1.8Å | |PDB= 1xxs |SIZE=350|CAPTION= <scene name='initialview01'>1xxs</scene>, resolution 1.8Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> | + | |LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=STE:STEARIC+ACID'>STE</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Phospholipase_A(2) Phospholipase A(2)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.4 3.1.1.4] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Phospholipase_A(2) Phospholipase A(2)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.4 3.1.1.4] </span> |
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1pa0|1PA0]], [[1pc9|1PC9]], [[1god|1GOD]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xxs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xxs OCA], [http://www.ebi.ac.uk/pdbsum/1xxs PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1xxs RCSB]</span> | ||
}} | }} | ||
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[[Category: Ward, R J.]] | [[Category: Ward, R J.]] | ||
[[Category: Watanabe, L.]] | [[Category: Watanabe, L.]] | ||
| - | [[Category: SO4]] | ||
| - | [[Category: STE]] | ||
[[Category: crystal structure]] | [[Category: crystal structure]] | ||
[[Category: dimer interface]] | [[Category: dimer interface]] | ||
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[[Category: stearic acid]] | [[Category: stearic acid]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:56:05 2008'' |
Revision as of 21:56, 30 March 2008
| |||||||
| , resolution 1.8Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||
| Activity: | Phospholipase A(2), with EC number 3.1.1.4 | ||||||
| Related: | 1PA0, 1PC9, 1GOD
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structural insights for fatty acid binding in a Lys49 phospholipase A2: crystal structure of myotoxin II from Bothrops moojeni complexed with stearic acid
Overview
The crystal structure of dimeric Lys49-phospholipase A2 myotoxin-II from Bothrops moojeni (MjTX-II) co-crystallized with stearic acid (C(18)H(36)O(2)) has been determined at a resolution of 1.8 A. The electron density maps permitted the unambiguous inclusion of six stearic acid molecules in the refinement. Two stearic acid molecules could be located in the substrate-binding cleft of each monomer in positions, which favor the interaction of their carboxyl groups with active site residues. The way of binding of stearic acids to this Lys49-PLA(2)s is analogous to phospholipids and transition state analogues to catalytically active PLA(2)s. Two additional stearic acid molecules were located at the dimer interface region, defining a hitherto unidentified acyl-binding site on the protein surface. The strictly conserved Lys122 for Lys49-PLA(2)s may play a fundamental role for stabilization of legend-protein complex. The comparison of MjTX-II/satiric acid complex with other Lys-PLA(2)s structures whose putative fatty acids were located at their active site is also analysed. Molecular details of the stearic acid/protein interactions provide insights to binding in group I/II PLA(2)s, and to the possible interactions of Lys49-PLA(2)s with target membranes.
About this Structure
1XXS is a Single protein structure of sequence from Bothrops moojeni. Full crystallographic information is available from OCA.
Reference
Structural insights for fatty acid binding in a Lys49-phospholipase A2: crystal structure of myotoxin II from Bothrops moojeni complexed with stearic acid., Watanabe L, Soares AM, Ward RJ, Fontes MR, Arni RK, Biochimie. 2005 Feb;87(2):161-7. PMID:15760708
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