1y6i
From Proteopedia
| Line 7: | Line 7: | ||
|ACTIVITY= | |ACTIVITY= | ||
|GENE= gun4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1143 Synechocystis sp.]) | |GENE= gun4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1143 Synechocystis sp.]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1y6i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y6i OCA], [http://www.ebi.ac.uk/pdbsum/1y6i PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1y6i RCSB]</span> | ||
}} | }} | ||
| Line 31: | Line 34: | ||
[[Category: porphyrin binding]] | [[Category: porphyrin binding]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:59:22 2008'' |
Revision as of 21:59, 30 March 2008
| |||||||
| , resolution 1.78Å | |||||||
|---|---|---|---|---|---|---|---|
| Gene: | gun4 (Synechocystis sp.) | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Synechocystis GUN4
Overview
In plants, the accumulation of the chlorophyll precursor Mg-protoporphyrin IX (Mg-Proto) in the plastid regulates the expression of a number of nuclear genes with functions related to photosynthesis. Analysis of the plastid-to-nucleus signaling activity of Mg-Proto in Arabidopsis thaliana led to the discovery of GUN4, a novel porphyrin-binding protein that also dramatically enhances the activity of Mg-chelatase, the enzyme that synthesizes Mg-Proto. GUN4 may also play a role in both photoprotection and the cellular shuttling of tetrapyrroles. Here we report a 1.78-A resolution crystal structure of Synechocystis GUN4, in which the porphyrin-binding domain adopts a unique three dimensional fold with a "cupped hand" shape. Biophysical and biochemical analyses revealed the specific site of interaction between GUN4 and Mg-Proto and the energetic determinants for the GUN4.Mg-Proto interaction. Our data support a novel protective function for GUN4 in tetrapyrrole trafficking. The combined structural and energetic analyses presented herein form the physical-chemical basis for understanding GUN4 biological activity, including its role in the stimulation of Mg-chelatase activity, as well as in Mg-Proto retrograde signaling.
About this Structure
1Y6I is a Single protein structure of sequence from Synechocystis sp.. Full crystallographic information is available from OCA.
Reference
Structure of the Mg-chelatase cofactor GUN4 reveals a novel hand-shaped fold for porphyrin binding., Verdecia MA, Larkin RM, Ferrer JL, Riek R, Chory J, Noel JP, PLoS Biol. 2005 May;3(5):e151. Epub 2005 Apr 26. PMID:15884974
Page seeded by OCA on Mon Mar 31 00:59:22 2008
