1j0r

From Proteopedia

(Difference between revisions)

Revision as of 09:55, 4 October 2017

Crystal structure of the replication termination protein mutant C110S

Structural highlights

1j0r is a 2 chain structure with sequence from "bacillus_globigii"_migula_1900 "bacillus globigii" migula 1900. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	1bm9, 1f4k
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[RTP_BACSU] Plays a role in DNA replication and termination (fork arrest mechanism). Two dimers of rtp bind to the two inverted repeat regions (IRI and IRII) present in the termination site. The binding of each dimer is centered on an 8 bp direct repeat.

Publication Abstract from PubMed

We report the structural and biophysical consequences of cysteine substitutions in the DNA-binding replication terminator protein (RTP) of Bacillus subtilis, that resulted in an optimised RTP mutant suitable for structural studies. The cysteine residue 110 was replaced with alanine, valine or serine. Protein secondary structure and stability (using circular dichroism spectropolarimetry), self-association (using analytical ultracentrifugation), and DNA-binding measurements revealed RTP.C110S to be the most similar mutant to wild-type RTP. The C110A and C110V.RTP mutants were less soluble, less stable and showed lower DNA-binding affinity. The structure of RTP.C110S, solved to 2.5A resolution using crystallographic methods, showed no major structural perturbation due to the mutation. Heteronuclear NMR spectroscopic studies revealed subtle differences in the electronic environment about the site of mutation. The study demonstrates the suitability of serine as a substitute for cysteine in RTP and the high sensitivity of protein behaviour to single amino acid substitutions.

The impact of single cysteine residue mutations on the replication terminator protein.,Vivian JP, Hastings AF, Duggin IG, Wake RG, Wilce MC, Wilce JA Biochem Biophys Res Commun. 2003 Oct 31;310(4):1096-103. PMID:14559228^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Vivian JP, Hastings AF, Duggin IG, Wake RG, Wilce MC, Wilce JA. The impact of single cysteine residue mutations on the replication terminator protein. Biochem Biophys Res Commun. 2003 Oct 31;310(4):1096-103. PMID:14559228

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1j0r"

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 ==Crystal structure of the replication termination protein mutant C110S==
 <StructureSection load='1j0r' size='340' side='right' caption='[[1j0r]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
@@ Line 4: / Line 5: @@
 <table><tr><td colspan='2'>[[1j0r]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_globigii"_migula_1900 "bacillus globigii" migula 1900]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1J0R OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1J0R FirstGlance]. <br>
 </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1bm9|1bm9]], [[1f4k|1f4k]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1j0r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1j0r OCA], [http://pdbe.org/1j0r PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1j0r RCSB], [http://www.ebi.ac.uk/pdbsum/1j0r PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1j0r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1j0r OCA], [http://pdbe.org/1j0r PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1j0r RCSB], [http://www.ebi.ac.uk/pdbsum/1j0r PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1j0r ProSAT]</span></td></tr>
 </table>
 == Function ==

1j0r

From Proteopedia

Revision as of 09:55, 4 October 2017

Crystal structure of the replication termination protein mutant C110S

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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