Signal recognition particle protein
From Proteopedia
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<StructureSection load='1e8o' size='350' side='right' scene='' caption='Human SRP9 (grey and pink) +SRP14 (green and yellow) complex with 7S RNA, GDP and sulfate [[1e8o]]'> | <StructureSection load='1e8o' size='350' side='right' scene='' caption='Human SRP9 (grey and pink) +SRP14 (green and yellow) complex with 7S RNA, GDP and sulfate [[1e8o]]'> | ||
'''Signal recognition particle''' (SRP) is a ribonucleoprotein which targets specific proteins to the endoplasmic reticulum (ER) in eukaryotes and the plasma membrane in prokaryotes. In eukaryotes SRP binds to the signal sequence of a newly synthesized polypeptide as it emerges from the ribosome. The binding allows for the coupling of the translation process to the translocation process. The SRP targets the nascent protein to the ER by docking into the [[Signal recognition particle receptor|SRP receptor]]<ref>PMID:15718142</ref>. The eukaryotic SRP which has GTPase activity, is composed of SRP9, SRP14<ref>PMID:7542942</ref>, SRP19<ref>PMID:11682607</ref>, SRP54<ref>PMID:9511762</ref>, SRP68, SRP72<ref>PMID:8388879</ref> and 7S RNA. The prokaryotic SRP is composed of FFH (SRP54-like) and 4.5S RNA. | '''Signal recognition particle''' (SRP) is a ribonucleoprotein which targets specific proteins to the endoplasmic reticulum (ER) in eukaryotes and the plasma membrane in prokaryotes. In eukaryotes SRP binds to the signal sequence of a newly synthesized polypeptide as it emerges from the ribosome. The binding allows for the coupling of the translation process to the translocation process. The SRP targets the nascent protein to the ER by docking into the [[Signal recognition particle receptor|SRP receptor]]<ref>PMID:15718142</ref>. The eukaryotic SRP which has GTPase activity, is composed of SRP9, SRP14<ref>PMID:7542942</ref>, SRP19<ref>PMID:11682607</ref>, SRP54<ref>PMID:9511762</ref>, SRP68, SRP72<ref>PMID:8388879</ref> and 7S RNA. The prokaryotic SRP is composed of FFH (SRP54-like) and 4.5S RNA. | ||
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**[[1kvn]], [[1kvv]] – SRP19 (mutant) – ''Archaeoglobus fulgidus'' – NMR<BR /> | **[[1kvn]], [[1kvv]] – SRP19 (mutant) – ''Archaeoglobus fulgidus'' – NMR<BR /> | ||
**[[1qb2]] – hSRP54 <br /> | **[[1qb2]] – hSRP54 <br /> | ||
| - | **[[2hug]] – AtSRP43 | + | **[[2hug]] – AtSRP43 chromodomain 2 + SRP54 M domain – ''Arabidopsis thaliana'' – NMR<BR /> |
| - | **[[1x32]] - AtSRP43 | + | **[[1x32]] - AtSRP43 chromodomain 1 – NMR<br /> |
| + | **[[5e4x]] - AtSRP43 chromodomain 3<br /> | ||
**[[3deo]] - AtSRP43 residues 85-267<br /> | **[[3deo]] - AtSRP43 residues 85-267<br /> | ||
**[[3ui2]] - AtSRP43 residues 84-327 + AtSRP54 RRKR motif<br /> | **[[3ui2]] - AtSRP43 residues 84-327 + AtSRP54 RRKR motif<br /> | ||
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**[[1qzx]] – SsSRP54 – ''Sulfolobus solfataricus''<br /> | **[[1qzx]] – SsSRP54 – ''Sulfolobus solfataricus''<br /> | ||
**[[4p3f]] – hSRP68 (mutant) – human<br /> | **[[4p3f]] – hSRP68 (mutant) – human<br /> | ||
| + | **[[5m72]], [[5wrv]] – hSRP68 + SRP72<br /> | ||
| + | **[[5wrw]] – hSRP72<br /> | ||
*Signal recognition particle complexes with RNA | *Signal recognition particle complexes with RNA | ||
| - | **[[1e8o]], [[1e8s]] - hSRP9 + hSRP14 + 7S RNA <br /> | + | **[[1e8o]], [[1e8s]], [[5aox]] - hSRP9 + hSRP14 + 7S RNA <br /> |
| + | **[[5m73]] - hSRP19 + hSRP68 + SRP72 + 7S RNA <br /> | ||
**[[1ry1]] - hSRP9 + hSRP14 + hSRP19 + hSRP54 + 7S RNA<br /> | **[[1ry1]] - hSRP9 + hSRP14 + hSRP19 + hSRP54 + 7S RNA<br /> | ||
**[[4uyj]], [[4uyk]] - hSRP9 + hSRP14 + RNA<br /> | **[[4uyj]], [[4uyk]] - hSRP9 + hSRP14 + RNA<br /> | ||
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**[[2pxb]], [[2pxd]], [[2pxe]], [[2pxf]], [[2pxk]], [[2pxl]], [[2pxq]], [[2pxq]], [[2pxt]], [[2pxu]], [[2pxv]] - EcSRP54-like C terminal + 4.5SRNA domain IV (mutant)<br /> | **[[2pxb]], [[2pxd]], [[2pxe]], [[2pxf]], [[2pxk]], [[2pxl]], [[2pxq]], [[2pxq]], [[2pxt]], [[2pxu]], [[2pxv]] - EcSRP54-like C terminal + 4.5SRNA domain IV (mutant)<br /> | ||
**[[2go5]] – dSRP19 + SRP54 + hSRP receptor α subunit + mSRP receptor b subunit + ribosomal proteins L23, L31, L35 + RNA – dog<br /> | **[[2go5]] – dSRP19 + SRP54 + hSRP receptor α subunit + mSRP receptor b subunit + ribosomal proteins L23, L31, L35 + RNA – dog<br /> | ||
| - | **[[2j28]] – EcSRP54-like + 70S ribosome – EM<BR /> | + | **[[4ue5]] – dSRP9 + SRP14 + SRP54 + SRP68 + RNA – Cryo EM<br /> |
| - | **[[2j37]] - | + | **[[2j28]] – EcSRP54-like + 70S ribosome – Cryo EM<BR /> |
| + | **[[2j37]] - SRP19 + SRP54 + ribosomal proteins L23, L31, L35 + RNA – wheat – Cryo EM<BR /> | ||
| + | **[[4ue4]] – SRP M domain + FTSQ signal sequence + RNA - ''Bacillus subtilis''<br /> | ||
*Other signal recognition particle complexes | *Other signal recognition particle complexes | ||
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**[[2j7p]] - TaSRP54-like NG domain + cell division protein FTSY + GMPPNP<BR /> | **[[2j7p]] - TaSRP54-like NG domain + cell division protein FTSY + GMPPNP<BR /> | ||
**[[3dep]] - AtSRP43 residues 85-267 + polypeptide<br /> | **[[3dep]] - AtSRP43 residues 85-267 + polypeptide<br /> | ||
| + | **[[5e4w]] - AtSRP43 chromodomain 2-3 + thioredoxin + ALB3 tail<br /> | ||
| + | **[[5l3r]] - AtSRP54 + cell division protein FTSY<br /> | ||
| + | **[[5l3s]] - SsSRP54 + cell division protein FTSY<br /> | ||
**[[3kl4]] – SsSRP54 residues 2-432 + polypeptide<br /> | **[[3kl4]] – SsSRP54 residues 2-432 + polypeptide<br /> | ||
| + | **[[5l3v]] - SsSRP54 + GDP<br /> | ||
**[[4c7o]] – EcSRP + FTSY + SRP RNA<br /> | **[[4c7o]] – EcSRP + FTSY + SRP RNA<br /> | ||
| + | **[[5l3q]] – hSRP54 + SRP receptor subunit α<br /> | ||
}} | }} | ||
== References == | == References == | ||
<references/> | <references/> | ||
[[Category:Topic Page]] | [[Category:Topic Page]] | ||
Revision as of 19:41, 4 October 2017
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3D structures of signal recognition particle
Updated on 04-October-2017
References
- ↑ Halic M, Beckmann R. The signal recognition particle and its interactions during protein targeting. Curr Opin Struct Biol. 2005 Feb;15(1):116-25. PMID:15718142 doi:http://dx.doi.org/10.1016/j.sbi.2005.01.013
- ↑ Bovia F, Fornallaz M, Leffers H, Strub K. The SRP9/14 subunit of the signal recognition particle (SRP) is present in more than 20-fold excess over SRP in primate cells and exists primarily free but also in complex with small cytoplasmic Alu RNAs. Mol Biol Cell. 1995 Apr;6(4):471-84. PMID:7542942
- ↑ Dean KA, von Ahsen O, Gorlich D, Fried HM. Signal recognition particle protein 19 is imported into the nucleus by importin 8 (RanBP8) and transportin. J Cell Sci. 2001 Oct;114(Pt 19):3479-85. PMID:11682607
- ↑ Gowda K, Black SD, Moeller I, Sakakibara Y, Liu MC, Zwieb C. Protein SRP54 of human signal recognition particle: cloning, expression, and comparative analysis of functional sites. Gene. 1998 Jan 30;207(2):197-207. PMID:9511762
- ↑ Lutcke H, Prehn S, Ashford AJ, Remus M, Frank R, Dobberstein B. Assembly of the 68- and 72-kD proteins of signal recognition particle with 7S RNA. J Cell Biol. 1993 Jun;121(5):977-85. PMID:8388879
