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| - | {{Large structure}}
| + | #REDIRECT [[5o7x]] This PDB entry is obsolete and replaced by 5o7x |
| - | ==Crystal structure of S. cerevisiae Core Factor at 3.2A resolution==
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| - | <StructureSection load='5n5x' size='340' side='right' caption='[[5n5x]], [[Resolution|resolution]] 3.20Å' scene=''>
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| - | == Structural highlights ==
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| - | <table><tr><td colspan='2'>[[5n5x]] is a 18 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5N5X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5N5X FirstGlance]. <br>
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| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5n5x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5n5x OCA], [http://pdbe.org/5n5x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5n5x RCSB], [http://www.ebi.ac.uk/pdbsum/5n5x PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5n5x ProSAT]</span></td></tr>
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| - | </table>
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| - | {{Large structure}}
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| - | == Function ==
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| - | [[http://www.uniprot.org/uniprot/RRN6_YEAST RRN6_YEAST]] Acts as component of the core factor (CF) complex which is essential for the initiation of rDNA transcription by RNA polymerase I. After binding of UAF (upstream activation factor) to an upstream element of the promoter, CF is recruited in a SPT15/TBP-dependent manner to form a preinitiation complex.<ref>PMID:8887672</ref> [[http://www.uniprot.org/uniprot/RRN11_YEAST RRN11_YEAST]] Acts as component of the core factor (CF) complex which is essential for the initiation of rDNA transcription by RNA polymerase I. After binding of UAF (upstream activation factor) to an upstream element of the promoter, CF is recruited in a SPT15/TBP-dependent manner to form a preinitiation complex.<ref>PMID:8887672</ref> [[http://www.uniprot.org/uniprot/RRN7_YEAST RRN7_YEAST]] Component of RNA polymerase I core factor complex (CF) that acts as a SUA7/TFIIB-like factor and plays a key role in multiple steps during transcription initiation such as pre-initiation complex (PIC) assembly and postpolymerase recruitment events in polymerase I (Pol I) transcription. Binds rDNA promoters and plays a role in Pol I recruitment. After binding of UAF (upstream activation factor) to an upstream element of the promoter, CF is recruited in a SPT15/TBP-dependent manner to form a pre-initiation complex.<ref>PMID:21921198</ref> <ref>PMID:21940764</ref> <ref>PMID:8887672</ref>
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| - | <div style="background-color:#fffaf0;">
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| - | == Publication Abstract from PubMed ==
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| - | Transcription initiation at the ribosomal RNA promoter requires RNA polymerase (Pol) I and the initiation factors Rrn3 and core factor (CF). Here, we combine X-ray crystallography and cryo-electron microscopy (cryo-EM) to obtain a molecular model for basal Pol I initiation. The three-subunit CF binds upstream promoter DNA, docks to the Pol I-Rrn3 complex, and loads DNA into the expanded active center cleft of the polymerase. DNA unwinding between the Pol I protrusion and clamp domains enables cleft contraction, resulting in an active Pol I conformation and RNA synthesis. Comparison with the Pol II system suggests that promoter specificity relies on a distinct "bendability" and "meltability" of the promoter sequence that enables contacts between initiation factors, DNA, and polymerase.
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| - | Structural Basis of RNA Polymerase I Transcription Initiation.,Engel C, Gubbey T, Neyer S, Sainsbury S, Oberthuer C, Baejen C, Bernecky C, Cramer P Cell. 2017 Mar 23;169(1):120-131.e22. doi: 10.1016/j.cell.2017.03.003. PMID:28340337<ref>PMID:28340337</ref>
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
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| - | <div class="pdbe-citations 5n5x" style="background-color:#fffaf0;"></div>
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| - | == References ==
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| - | <references/>
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| - | __TOC__
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| - | </StructureSection>
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| - | [[Category: Baejen, C]]
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| - | [[Category: Bernecky, C]]
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| - | [[Category: Cramer, P]]
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| - | [[Category: Engel, C]]
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| - | [[Category: Gubbey, T]]
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| - | [[Category: Neyer, S]]
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| - | [[Category: Oberthuer, C]]
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| - | [[Category: Sainsbury, S]]
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| - | [[Category: Core factor]]
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| - | [[Category: Rna polymerase i initiation]]
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| - | [[Category: Transcription]]
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