1ybk
From Proteopedia
| Line 4: | Line 4: | ||
|PDB= 1ybk |SIZE=350|CAPTION= <scene name='initialview01'>1ybk</scene>, resolution 1.45Å | |PDB= 1ybk |SIZE=350|CAPTION= <scene name='initialview01'>1ybk</scene>, resolution 1.45Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= | + | |LIGAND= <scene name='pdbligand=BEQ:N-(CARBOXYMETHYL)-N,N-DIMETHYL-3-[(1-OXODODECYL)AMINO]-1-PROPANAMINIUM+INNER+SALT'>BEQ</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1fe6|1FE6]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ybk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ybk OCA], [http://www.ebi.ac.uk/pdbsum/1ybk PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ybk RCSB]</span> | ||
}} | }} | ||
| Line 23: | Line 26: | ||
[[Category: Staphylothermus marinus]] | [[Category: Staphylothermus marinus]] | ||
[[Category: Stetefeld, J.]] | [[Category: Stetefeld, J.]] | ||
| - | [[Category: BEQ]] | ||
| - | [[Category: SO4]] | ||
[[Category: right hand coiled coil]] | [[Category: right hand coiled coil]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:01:56 2008'' |
Revision as of 22:02, 30 March 2008
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| , resolution 1.45Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||
| Related: | 1FE6
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
RHCC cocrystallized with CAPB
Overview
Crystals of excellent quality are a prerequisite for high-resolution X-ray data. However, in refinement protocols of crystallization conditions it is often difficult to obtain the right combination of, for example, protein concentration, drop size, temperature and additives. A novel approach for optimizing crystal contacts in a most favourable fashion by performing crystallization setups with the zwitterionic surfactant cocoamidoproylbetaine (CAPB) is introduced. In the presence of this surfactant, highly diffracting crystals were obtained. Here, data from a right-handed coiled coil (RHCC) in complex with CAPB at 1.4 A resolution are presented. The addition of CAPB using otherwise identical crystallization conditions and the same X-ray source caused an improvement in resolution from 2.9 to 1.4 A.
About this Structure
1YBK is a Single protein structure of sequence from Staphylothermus marinus. Full crystallographic information is available from OCA.
Reference
Favourable mediation of crystal contacts by cocoamidopropylbetaine (CAPB)., Ozbek S, Muller JF, Figgemeier E, Stetefeld J, Acta Crystallogr D Biol Crystallogr. 2005 Apr;61(Pt 4):477-80. Epub 2005, Mar 24. PMID:15805603
Page seeded by OCA on Mon Mar 31 01:01:56 2008
